Medical Microbiology and Immunology

, Volume 199, Issue 1, pp 11–25

Cellular immune response to Mycobacterium tuberculosis-specific antigen culture filtrate protein-10 in south India

Authors

  • Madhan Kumar
    • Department of ImmunologyTuberculosis Research Centre (ICMR)
  • Jagadish C. Sundaramurthi
    • Biomedical Informatics CentreTuberculosis Research Centre (ICMR)
  • Narinder K. Mehra
    • Department of Transplant Immunology and ImmunogeneticsAll India Institute of Medical Sciences
  • Gurvinder Kaur
    • Department of Transplant Immunology and ImmunogeneticsAll India Institute of Medical Sciences
    • Department of ImmunologyTuberculosis Research Centre (ICMR)
Original Investigation

DOI: 10.1007/s00430-009-0129-2

Cite this article as:
Kumar, M., Sundaramurthi, J.C., Mehra, N.K. et al. Med Microbiol Immunol (2010) 199: 11. doi:10.1007/s00430-009-0129-2

Abstract

The Mycobacterium tuberculosis (M. tuberculosis)-specific culture filtrate protein-10 (CFP-10) is highly recognized by M. tuberculosis infected subjects. In the present study, the proliferative response and IFN-γ secretion was found for C-terminal peptides of the protein (Cfp651–70, Cfp761–80, Cfp871–90, and Cfp981–100). The alleles HLA DRB1 *04 and HLA DRB1 *10 recognized the C-terminal peptides Cfp7, Cfp8, and Cfp9 in HHC. Cfp6 was predominantly recognized by the alleles HLA DRB1 *03 and HLA DRB1 *15 by PTB. The minimal nonameric epitopes from the C-terminal region were CFP-1056–64 and CFP-1076–84. These two peptides deserve attention for inclusion in a vaccine against tuberculosis in this region.

Keywords

CFP-10HLA analysisEpitope mappingOverlapping peptidesProliferationInterferon gammaIn silico CFP-10IL-4ELISATuberculosis

Supplementary material

430_2009_129_MOESM1_ESM.doc (170 kb)
Supplementary material 1 (DOC 170 kb)

Copyright information

© Springer-Verlag 2009