Medical Microbiology and Immunology

, Volume 197, Issue 1, pp 21–27

Fetal calf serum inhibits virus genome expression in Madin-Darby canine kidney cells persistently infected with influenza A virus

  • Md. Jaber Hossain
  • Isamu Mori
  • Li Dong
  • Beixing Liu
  • Yoshinobu Kimura
Original Investigation

DOI: 10.1007/s00430-007-0054-1

Cite this article as:
Hossain, M.J., Mori, I., Dong, L. et al. Med Microbiol Immunol (2008) 197: 21. doi:10.1007/s00430-007-0054-1

Abstract

A cell line of Madin-Darby canine kidney (MDCK) cells persistently infected with human influenza A virus has been established and designated as MDCK-IVpi cells. Production of progeny virus in MDCK-IVpi cells was suppressed when the cells were incubated in the presence of 10% fetal calf serum (FCS). FCS impaired virus mRNA synthesis in MDCK-IVpi cells, which resulted in a scarcity of virus proteins for virion formation. However, MDCK-IVpi cells well supported the growth of superinfecting heterologous influenza viruses, even in the presence of FCS. A certain fetuin-like substance in FCS might be responsible for the observed inhibition of virus replication.

Keywords

Influenza virus Gene expression Blood serum Virus carrier culture 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Md. Jaber Hossain
    • 1
  • Isamu Mori
    • 1
    • 2
  • Li Dong
    • 1
  • Beixing Liu
    • 1
  • Yoshinobu Kimura
    • 1
    • 3
  1. 1.Department of MicrobiologyFukui University School of MedicineFukuiJapan
  2. 2.Department of Microbiology and ImmunologyAichi Medical University School of MedicineAichiJapan
  3. 3.Department of Medical TechnologyGifu University of Medical ScienceSeki, GifuJapan