Medical Microbiology and Immunology

, Volume 196, Issue 2, pp 103–114

Peptidomic analysis of human peripheral monocytes persistently infected by Chlamydia trachomatis

Authors

    • Division of Rheumatology, Department of Internal MedicineHannover Medical School
    • Department of Rheumatology (OE 6850)Hannover Medical School
  • Annette Busmann
    • Digilab BioVisioN GmbH
  • Harald Tammen
    • Digilab BioVisioN GmbH
  • Christoph Menzel
    • Digilab BioVisioN GmbH
  • Thomas Möhring
    • Digilab BioVisioN GmbH
  • Nicolas Le Yondre
    • Digilab BioVisioN GmbH
  • Cornelia Schmidt
    • Division of Rheumatology, Department of Internal MedicineHannover Medical School
  • Peter Schulz-Knappe
    • Digilab BioVisioN GmbH
  • Henning Zeidler
    • Division of Rheumatology, Department of Internal MedicineHannover Medical School
  • Hartmut Selle
    • Digilab BioVisioN GmbH
  • Lars Köhler
    • Division of Rheumatology, Department of Internal MedicineHannover Medical School
Original Investigation

DOI: 10.1007/s00430-006-0033-y

Cite this article as:
Krausse-Opatz, B., Busmann, A., Tammen, H. et al. Med Microbiol Immunol (2007) 196: 103. doi:10.1007/s00430-006-0033-y

Abstract

Peptidomic analysis using Differential Peptide Display (DPD) of human peripheral blood mononuclear cells (PBMC) mock-infected or persistently infected by Chlamydia trachomatis (CT) revealed 10 peptides, expressed upon CT infection. Analysis of these 10 candidates by tandem mass spectrometry enabled the determination of seven candidates as fragments from the precursors (I) ferritin heavy chain subunit, (II) HLA class II histocompatibility antigen, (III) vimentin, (IV) indoleamine 2,3-dioxygenase, (V and VI) pre-B cell enhancing factor (PBEF), and (VII) Interleukin-8 (CXCL8). The identified candidates proved the presence of anti-bacterial and immunologically active monocytic proteins after CT infection.

Keywords

Chlamydia trachomatis Monocytes Peptidomics

Copyright information

© Springer-Verlag 2007