Original Investigation

Medical Microbiology and Immunology

, Volume 196, Issue 2, pp 103-114

Peptidomic analysis of human peripheral monocytes persistently infected by Chlamydia trachomatis

  • Birgit Krausse-OpatzAffiliated withDivision of Rheumatology, Department of Internal Medicine, Hannover Medical SchoolDepartment of Rheumatology (OE 6850), Hannover Medical School Email author 
  • , Annette BusmannAffiliated withDigilab BioVisioN GmbH
  • , Harald TammenAffiliated withDigilab BioVisioN GmbH
  • , Christoph MenzelAffiliated withDigilab BioVisioN GmbH
  • , Thomas MöhringAffiliated withDigilab BioVisioN GmbH
  • , Nicolas Le YondreAffiliated withDigilab BioVisioN GmbH
  • , Cornelia SchmidtAffiliated withDivision of Rheumatology, Department of Internal Medicine, Hannover Medical School
  • , Peter Schulz-KnappeAffiliated withDigilab BioVisioN GmbH
  • , Henning ZeidlerAffiliated withDivision of Rheumatology, Department of Internal Medicine, Hannover Medical School
    • , Hartmut SelleAffiliated withDigilab BioVisioN GmbH
    • , Lars KöhlerAffiliated withDivision of Rheumatology, Department of Internal Medicine, Hannover Medical School

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Abstract

Peptidomic analysis using Differential Peptide Display (DPD) of human peripheral blood mononuclear cells (PBMC) mock-infected or persistently infected by Chlamydia trachomatis (CT) revealed 10 peptides, expressed upon CT infection. Analysis of these 10 candidates by tandem mass spectrometry enabled the determination of seven candidates as fragments from the precursors (I) ferritin heavy chain subunit, (II) HLA class II histocompatibility antigen, (III) vimentin, (IV) indoleamine 2,3-dioxygenase, (V and VI) pre-B cell enhancing factor (PBEF), and (VII) Interleukin-8 (CXCL8). The identified candidates proved the presence of anti-bacterial and immunologically active monocytic proteins after CT infection.

Keywords

Chlamydia trachomatis Monocytes Peptidomics