Brain Structure and Function

, Volume 219, Issue 1, pp 395–406

Differential regulation of parvalbumin and calretinin interneurons in the prefrontal cortex during adolescence

  • Adriana Caballero
  • Eden Flores-Barrera
  • Daryn K. Cass
  • Kuei Y. Tseng
Original Article

DOI: 10.1007/s00429-013-0508-8

Cite this article as:
Caballero, A., Flores-Barrera, E., Cass, D.K. et al. Brain Struct Funct (2014) 219: 395. doi:10.1007/s00429-013-0508-8

Abstract

Determining the normal developmental trajectory of individual GABAergic components in the prefrontal cortex (PFC) during the adolescent transition period is critical because local GABAergic interneurons are thought to play an important role in the functional maturation of cognitive control that occurs in this developmental window. Based on the expression of calcium-binding proteins, three distinctive subtypes of interneurons have been identified in the PFC: parvalbumin (PV)-, calretinin (CR)-, and calbindin (CB)-positive cells. Using biochemical and histochemical measures, we found that the protein level of PV is lowest in juveniles [postnatal days (PD) 25–35] and increases during adolescence (PD 45–55) to levels similar to those observed in adulthood (PD 65–75). In contrast, the protein expression of CR is reduced in adults compared to juvenile and adolescent animals, whereas CB levels remain mostly unchanged across the developmental window studied here. Semi-quantitative immunostaining analyses revealed that the periadolescent upregulation of PV and the loss of the CR signal appear to be attributable to changes in PV- and CR-positive innervation, which are dissociable from the trajectory of PV- and CR-positive cell number. At the synaptic level, our electrophysiological data revealed that a developmental facilitation of spontaneous glutamatergic synaptic inputs onto PV-positive/fast-spiking interneurons parallels the increase in prefrontal PV signal during the periadolescent transition. In contrast, no age-dependent changes in glutamatergic transmission were observed in PV-negative/non fast-spiking interneurons. Together, these findings emphasize that GABAergic inhibitory interneurons in the PFC undergo a dynamic, cell type-specific remodeling during adolescence and provide a developmental framework for understanding alterations in GABAergic circuits that occur in psychiatric disorders.

Keywords

Interneurons Calcium-binding proteins Prefrontal cortex Fast spiking Non-fast spiking 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Adriana Caballero
    • 1
  • Eden Flores-Barrera
    • 1
  • Daryn K. Cass
    • 1
  • Kuei Y. Tseng
    • 1
  1. 1.Department of Cellular and Molecular PharmacologyThe Chicago Medical School at Rosalind Franklin University of Medicine and ScienceNorth ChicagoUSA

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