Brain Structure and Function

, Volume 216, Issue 3, pp 201–218

Laminar distribution of neurotransmitter receptors in different reeler mouse brain regions

  • Christian M. Cremer
  • Joachim H. R. Lübke
  • Nicola Palomero-Gallagher
  • Karl Zilles
Original Article

DOI: 10.1007/s00429-011-0303-3

Cite this article as:
Cremer, C.M., Lübke, J.H.R., Palomero-Gallagher, N. et al. Brain Struct Funct (2011) 216: 201. doi:10.1007/s00429-011-0303-3

Abstract

Mapping of multiple receptors of neurotransmitters provides insight into the spatial distribution of neurotransmission-relevant molecules in the cerebral cortex. During development, lack of reelin leads to impaired migration, disturbed lamination of the hippocampus and inverted neocortical layering. In the adult, reelin may regulate synaptic plasticity by modulating neurotransmitter receptor function. Using quantitative in vitro receptor autoradiography, different receptors, in particular, the binding site densities and laminar distribution of various glutamate, GABA, muscarinic and nicotinic acetylcholine, serotonin, dopamine and adenosine receptors, were analyzed in cortical and subcortical structures of reeler and wild-type brains. Differential changes in the laminar distribution, maximum binding capacity (Bmax) and regional density of neurotransmitter receptors were found in the reeler brain. A decrease of whole brain Bmax was found for adenosine A1 and GABAA receptors. In the forebrain, several binding sites were differentially up- or down-regulated (kainate, A1, benzodiazepine, 5-HT1, M2, α1 and α2). In the hippocampus, a significant decrease of GABAB, 5-HT1 and \( {\text{A}}_1^{\prime} \) receptors were observed. The density of M2 receptors increased, while other receptors remained unchanged. In the neocortex, some receptors demonstrated an obviously inverted laminar distribution (AMPA, kainate, NMDA, GABAB, 5-HT1, M1, M3, nAch), while the distribution of others (A1, GABAA, benzodiazepine, 5-HT2, muscarinic M2, adrenergic α1, α2) seemed to be less affected. Thus, the laminar receptor distribution is modulated by the developmental impairment and suggests and reflects partially the laminar inversion in reeler mice.

Keywords

Reeler mouseQuantitative receptor autoradiographyNeurotransmitter receptor mappingCortical organizationSynaptic transmission and plasticity

Supplementary material

429_2011_303_MOESM1_ESM.eps (24.2 mb)
Supplementary material 1 (EPS 24752 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Christian M. Cremer
    • 1
    • 2
  • Joachim H. R. Lübke
    • 1
    • 3
    • 4
  • Nicola Palomero-Gallagher
    • 1
  • Karl Zilles
    • 1
    • 2
    • 3
  1. 1.Institute of Neuroscience and Medicine INM-2Research Centre Jülich GmbHJülichGermany
  2. 2.C. & O. Vogt Institute for Brain ResearchHeinrich-Heine-University DüsseldorfDüsseldorfGermany
  3. 3.JARA Translational Brain MedicineJülich, AachenGermany
  4. 4.Department of Psychiatry and Psychotherapy, Medical FacultyRWTH/University Hospital AachenAachenGermany