Anatomy and Embryology

, Volume 208, Issue 2, pp 145–150

Involvement of p27(KIP1) in proliferation of the retinal pigment epithelium and ciliary body

  • Kazuhiko Yoshida
  • Keiko Nakayama
  • Satoru Kase
  • Hiroyasu Nagahama
  • Takayuki Harada
  • Hiromi Ikeda
  • Chikako Harada
  • Junko Imaki
  • Kazuhiro Ohgami
  • Kenji Shiratori
  • Shigeaki Ohno
  • Shinzo Nishi
  • Keiichi I. Nakayama
Original Article

DOI: 10.1007/s00429-004-0382-5

Cite this article as:
Yoshida, K., Nakayama, K., Kase, S. et al. Anat Embryol (2004) 208: 145. doi:10.1007/s00429-004-0382-5

Abstract

According to observations in various cell lines, elimination of the cyclin-dependent kinase inhibitor p27(KIP1) during the late G1 phase of the cell cycle is required for progression to the S phase. Eyes from C57BL/6 mice at embryonic days 13, 14, and 18, and at 4 weeks of age, were analyzed by a bromodeoxyuridine cell proliferation assay and by immunocytochemistry using anti-p27(KIP1) antibody. On embryonic days 14 and 18, p27(KIP1) was detected in the ciliary body. This protein also was detected in the nuclei of the many cells of the retinal pigment epithelium on embryonic day 18, and was present in all such cells at 4 weeks of age. When p27(KIP1)-/- knockout and control mice were injected with bromodeoxyuridine between postnatal days 7 and 10 and analyzed on day 11, positive cells were abundant in the retinal pigment epithelium and the ciliary body of p27(KIP1)-/- mice, whereas few cells were positive in control mice. By fluorescent nuclear staining in whole mounts of retinal pigment epithelium at 12 weeks of age, more nuclei were present in p27(KIP1)-/- than in the wild-type mice. These results suggest that p27(KIP1) was involved in regulation of proliferation in the RPE and the ciliary body.

Keywords

Retinal pigment epitheliump27(KIP1)Ciliary body

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Kazuhiko Yoshida
    • 1
  • Keiko Nakayama
    • 2
    • 7
  • Satoru Kase
    • 1
  • Hiroyasu Nagahama
    • 6
  • Takayuki Harada
    • 1
    • 3
  • Hiromi Ikeda
    • 4
  • Chikako Harada
    • 1
    • 3
  • Junko Imaki
    • 5
  • Kazuhiro Ohgami
    • 1
  • Kenji Shiratori
    • 1
  • Shigeaki Ohno
    • 1
  • Shinzo Nishi
    • 4
  • Keiichi I. Nakayama
    • 6
  1. 1.Department of OphthalmologyHokkaido University School of MedicineSapporoJapan
  2. 2.Division of Developmental Genetics, Center for Translational and Advanced Animal Research on Human DiseaseTohoku University Graduate School of MedicineMiyagiJapan
  3. 3.Department of Molecular NeurobiologyTokyo Metropolitan Institute of NeuroscienceTokyo 183-8526Japan
  4. 4.Department of BiochemistryHokkaido University School of MedicineSapporoJapan
  5. 5.Department of AnatomyNippon Medical CollegeTokyoJapan
  6. 6.Department of Molecular and Cellular BiologyMedical Institute of Bioregulation, Kyushu UniversityFukuokaJapan
  7. 7.Laboratory of Embryonic and Genetic EngineeringMedical Institute of Bioregulation, Kyushu UniversityFukuokaJapan