Anatomy and Embryology

, Volume 207, Issue 1, pp 35–44

Mandibular coronoid process in parathyroid hormone-related protein-deficient mice shows ectopic cartilage formation accompanied by abnormal bone modeling

  • Shunichi Shibata
  • Naoto Suda
  • Kenji Fukada
  • Kimie Ohyama
  • Yasuo Yamashita
  • Vicki E. Hammond
Original Article

DOI: 10.1007/s00429-003-0325-6

Cite this article as:
Shibata, S., Suda, N., Fukada, K. et al. Anat Embryol (2003) 207: 35. doi:10.1007/s00429-003-0325-6

Abstract

Parathyroid hormone-related protein (PTHrP) null mutant mice were analyzed to investigate an additional role for PTHrP in cell differentiation. We found ectopic cartilage formation in the mandibular coronoid process in newborn mice. While many previous studies involving PTHrP gene knockout mouse have shown that the cartilage in various regions becomes smaller, this is the first report showing an "increase" of cartilage volume. Investigations of mandibular growth using normal mice indicated that coronoid secondary cartilage never formed from E 15 to d 4, but small amount of cartilage temporally formed at d 7, and this also applies to PTHrP-wild type mice. Therefore, PTHrP deficiency consequently advanced the secondary cartilage formation, which is a novel role of PTHrP in chondrocyte differentiation. In situ hybridization of matrix proteins showed that this coronoid cartilage had characteristics of the lower hypertrophic cell zone usually present at the site of endochondral bone formation and/or "chondroid bone" occasionally found in distraction osteogenesis. In addition, the coronoid process in the PTHrP-deficient mouse also showed abnormal expansion of bone marrow and an increase in the number of multinucleated osteoclasts, an indication of abnormal bone modeling. These results indicate that PTHrP is involved in bone modeling as well as in chondrocyte differentiation. In situ hybridization of matrix protein mRNAs in the abnormal mandibular condylar cartilage revealed that this cartilage was proportionally smaller, supporting previous immunohistochemical results.

Keywords

ChondrocytesOsteoclastsIn-situ hybridizationImmunohistochemistry

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Shunichi Shibata
    • 1
  • Naoto Suda
    • 2
  • Kenji Fukada
    • 2
  • Kimie Ohyama
    • 2
  • Yasuo Yamashita
    • 1
  • Vicki E. Hammond
    • 3
  1. 1.Maxillofacial Anatomy, Department of Maxillofacial Biology, Graduate SchoolTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Maxillofacial Orthognatics, Department of Maxillofacial Reconstruction and Function, Graduate SchoolTokyo Medical and Dental UniversityTokyo Japan
  3. 3.Howard Florey Institute of Experimental Physiology and MedicineUniversity of MelbourneParkvilleAustralia