Virchows Archiv

, Volume 439, Issue 1, pp 78–84

Constrictive bronchiolitis obliterans

Characterisation of fibrogenesis and lysyl oxidase expression patterns
  • N. Streichenberger
  • S. Peyrol
  • F. Philit
  • R. Loire
  • P. Sommer
  • J. Cordier
Original Article

DOI: 10.1007/s004280100402

Cite this article as:
Streichenberger, N., Peyrol, S., Philit, F. et al. Virchows Arch (2001) 439: 78. doi:10.1007/s004280100402

Abstract.

The process leading to irreversible fibrotic constriction of the bronchioles was studied in two cases of bronchiolitis obliterans (BO) after bone marrow transplantation. Because lysyl oxidase (LOX) is the main collagen cross-linking enzyme that might account for irreversible fibrosis, its expression was studied together with expression of extracellular matrix (ECM) proteins. Characteristic types of lesions could be distinguished on the basis of histological and immunohistological criteria. An inflammatory stage was characterised by infiltration restricted to the bronchioles by lymphocytes and dendritic cells. A fibro-inflammatory stage was characterised by the coexistence of a persistent immune cellular lesion pattern with further focal modelling of a sub-epithelial neo-synthesised connective matrix. LOX expression was observed at the tips of intra-luminal fibrotic protrusions, together with tenascin and cellular fibronectin. A fibrotic stage was characterised by dense ECM deposits spreading throughout the peri-bronchiolar connective tissue, resulting in bronchiole obliteration and final disappearance. In contrast to reversible cases of fibrosis, persistence of long-term LOX expression reflecting continuing fibrosing activity might account for the irreversible status of BO. Our two cases illustrated that, at inflammatory and fibro-inflammatory stages, BO may be stabilised by immunosuppressive treatment, while the persistence of LOX expression in the fibrotic stage might correspond to a disease that becomes irreversible and fatal.

Bronchiolitis obliterans Lung fibrosis Lysyl oxidase Extracellular matrix Allogenic bone marrow transplantation 

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • N. Streichenberger
    • 1
  • S. Peyrol
    • 2
  • F. Philit
    • 4
  • R. Loire
    • 5
  • P. Sommer
    • 3
  • J. Cordier
    • 4
  1. 1.Service de Neuropathologie, Hôpital Neurologique, BP Lyon-Montchat 69 394 Lyon cedex 03, FranceFrance
  2. 2.Centre Commun de Microscopie Electronique, University Claude Bernard, Lyon, FranceFrance
  3. 3.Institut de Biologie et Chimie des Protéines, CNRS UPR-UMR 5096, Lyon, FranceFrance
  4. 4.Service de Pneumologie, Hôpital Cardiovasculaire et Pneumologique, Lyon, FranceFrance
  5. 5.Service d'Anatomie Pathologique de l'Hôpital Cardiovasculaire et Pneumologique, Lyon FranceFrance