Virchows Archiv

, Volume 434, Issue 1, pp 63–69

Expression patterns of type II pneumocyte apical surface glycoconjugates in lung adenocarcinoma cells

  • T. Hachiya
  • T. Honda
  • Keishi Kubo
  • Morie Sekiguchi
ORIGINAL ARTICLE

DOI: 10.1007/s004280050306

Cite this article as:
Hachiya, T., Honda, T., Kubo, K. et al. Virchows Archiv (1999) 434: 63. doi:10.1007/s004280050306

Abstract

 Monoclonal antibodies and lectins were used to examine the expression patterns of apical membrane oligosaccharide sequences specific to type II pneumocytes in atypical adenomatous hyperplasia (AAH) and lung cancer. Atypical cells of AAH and papillary adenocarcinoma cells expressed abundant sialyl Thomsen-Friedenreich (TF) antigen: this was not observed in acinar adenocarcinoma, bronchioloalveolar carcinoma with mucin production or squamous cell carcinoma. Sialyl Tn antigens was also detected on a few cells in AAH and papillary adenocarcinomas. Asialo TF and Tn antigen were not observed on the surface of carcinoma cells of any type. Alpha(α)2,3-linked sialic acids predominated in type II pneumocyte, AAH and papillary adenocarcinoma, whereas ciliated columnar cells expressed α2,6-linked sialic acids. Lewisx and sialyl Lewisx antigens capped the TF antigen in both O- and N-linked side chains on the surface of AAH and papillary adenocarcinoma cells, but were not expressed by type II pneumocytes. The findings demonstrate that papillary adenocarcinoma cells resemble type II pneumocytes in that they express abundant sialyl TF surface antigen, but they also express TF-related antigens not found in type II pneumocytes. Apical surface glycoconjugates of AAH have structural characteristics shared by both type II pneumocytes and papillary adenocarcinoma cells.

Key words Lung adenocarcinomaType II neumocyteThomsen-Friedenreich antigenMaclura pomifera agglutininSialyl Lewis

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • T. Hachiya
    • 1
  • T. Honda
    • 2
  • Keishi Kubo
    • 1
  • Morie Sekiguchi
    • 1
  1. 1.The First Department of Internal Medicine, Shinshu University School of Medicine, 3-1-1, Asahi, Matsumoto 390-8621, JapanJP
  2. 2.Department of Laboratory Medicine, Shinshu University School of Medicine, Matsumoto 390-8621, Japan Tel.: +81-263-35-4600 (ext. 6536) Fax: +81-263-34-5316JP