Virchows Archiv

, Volume 431, Issue 4, pp 249–256

Characteristics of rhabdomyosarcoma cell lines derived from uterine carcinosarcomas

  • M. Emoto
  • H. Iwasaki
  • K. Oshima
  • M. Kikuchi
  • Y. Kaneko
  • T. Kawarabayashi
ORIGINAL ARTICLE

DOI: 10.1007/s004280050096

Cite this article as:
Emoto, M., Iwasaki, H., Oshima, K. et al. Virchows Archiv (1997) 431: 249. doi:10.1007/s004280050096
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Abstract

 Rhabdomyosarcoma (RMS) is occasionally found in the female genital tract, and mostly appears as one of the heterologous mesenchymal components in uterine carcinosarcoma designated as malignant mixed müllerian tumour (MMMT). We examined the biological properties of a pure rhabdomyosarcoma (RMS) cell line designated FU-MMT-3, which was newly established from a surgical specimen taken from a patient with uterine MMMT. We also evaluated c-myc and MYCN gene amplification in three RMS cell lines (including FU-MMT-3) derived from three MMMTs by Southern blot analysis. FU-MMT-3 cells were propagated continuously for 57 serial passages over a 2-year period in vitro. FU-MMT-3 was able to produce tumours demonstrating pure RMS in athymic nude mice. Cytogenetically, FU-MMT-3 showed a triploidy pattern, with complex karyotypic abnormalities including trisomy of chromosome 8. All three RMS cell lines, including FU-MMT-3, showed amplification of the c-myc gene (approximately fourfold to eightfold), while no cell lines demonstrated MYCN gene amplification. FU-MMT-3 is considered to provide a useful system for the study of the biological behaviour of RMS in MMMTs. Extra copies of chromosome 8 and c-myc gene amplification may be associated with the rhabdomyoblastic differentiation in MMMT.

Key words Malignant mixed müllerian tumour Carcinosarcoma Rhabdomyosarcoma Tissue culture Cytogenetics 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • M. Emoto
    • 1
  • H. Iwasaki
    • 2
  • K. Oshima
    • 2
  • M. Kikuchi
    • 2
  • Y. Kaneko
    • 2
  • T. Kawarabayashi
    • 1
  1. 1.Department of Obstetrics and Gynecology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814–80, Japan Tel.: (81) 92-801-1011, Fax (81) 92-865-4114JP
  2. 2.Department of Pathology, Fukuoka University School of Medicine, Fukuoka, JapanJP
  3. 3.Department of Laboratory Medicine, Saitama Cancer Center, Saitama, JapanJP

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