Virchows Archiv

, Volume 437, Issue 2, pp 173–179

Simple mucin-type carbohydrate antigens (Tn, sialosyl-Tn, T and sialosyl-T) and gp 230 mucin-like glycoprotein are candidate markers for neoplastic transformation of the human cervix

  • Carla Carrilho
  • Martha Cantel
  • Patricia Gouveia
  • Leonor David
Original Article

DOI: 10.1007/s004280000218

Cite this article as:
Carrilho, C., Cantel, M., Gouveia, P. et al. Virchows Archiv (2000) 437: 173. doi:10.1007/s004280000218

Abstract 

Mucins and simple mucin-type carbohydrates are cancer-associated antigens in several human tumors. Expression of Tn, sialosyl-Tn, Thomsen-Friedenreich (T), sialosyl-T and of a recently identified mucin-like glycoprotein (gp230) has not yet been thoroughly investigated in human cervix carcinogenesis. In the present study sections from normal cervix (n=10), CIN III lesions (n=10), and invasive carcinomas (n=47) were evaluated immunohistochemically using monoclonal antibodies. In normal cervix there was: cytoplasmatic expression of Tn in 1 case (10%); membranous expression of STn in 8 cases (80%); no expression of T and cytoplasmatic expression of ST in 1 case (10%); gp 230 was expressed in all cases with a membranous pattern. In CIN III lesions there was cytoplasmatic and membranous expression of Tn in 3 cases (30%) and of STn in 9 cases (90%); T and ST were not expressed; gp 230 was expressed in 5 cases (50%) both in the cytoplasm and at the cell membrane. In invasive carcinomas we observed Tn expression in 30 cases (63.8%) and STn in 31 cases (66%); T antigen was not expressed; expression of both ST and gp 230 in 24 cases (51.1%); all antigens showed membranous and cytoplasmatic staining. Our results show that Tn and ST are good markers of invasive carcinomas of the human cervix. We have also shown that loss of expression of the mucin-like glycoprotein gp 230 is associated with malignant transformation at a preinvasive stage.

Key words Cervix carcinomaSimple mucin-type carbohydratesTumor markersMucinsTn antigen

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Carla Carrilho
    • 1
  • Martha Cantel
    • 1
  • Patricia Gouveia
    • 2
  • Leonor David
    • 2
  1. 1.Department of Pathology, Medical School, Eduardo Mondlane University, Maputo, MozambiqueMZ
  2. 2.IPATIMUP – Institute of Molecular Pathology and Immunology, University of Porto, 4200 Porto, PortugalPT
  3. 3.Medical Faculty of the University of Porto, 4200 Porto, PortugalPT
  4. 4.Instituto de Patologia e Imunologia Molecular da Universidade do Porto (IPATIMUP) – Rua Dr. Roberto Frias, s/n, 4200, Porto, Portugal e-mail: leonor.david@ipatimup.pt Tel.: +351-22-5570700, Fax: +351-22-5570799PT