Virchows Archiv

, Volume 463, Issue 1, pp 85–92

Predicting dedifferentiation in liposarcoma: a proteomic approach

Authors

  • Colt M. McClain
    • Department of Pathology, Microbiology and ImmunologyVanderbilt University Medical Center
  • David B. Friedman
    • Department of Biochemistry and Mass Spectrometry Research CenterVanderbilt University Medical Center
  • Tahar Hajri
    • Department of SurgeryVanderbilt University Medical Center
  • Cheryl M. Coffin
    • Department of Pathology, Microbiology and ImmunologyVanderbilt University Medical Center
    • Department of Pathology, Microbiology and ImmunologyVanderbilt University Medical Center
Original Article

DOI: 10.1007/s00428-013-1416-2

Cite this article as:
McClain, C.M., Friedman, D.B., Hajri, T. et al. Virchows Arch (2013) 463: 85. doi:10.1007/s00428-013-1416-2

Abstract

There are no known morphologic characteristics, cytogenetic aberrations, or molecular alterations predictive of dedifferentiation in liposarcomas. Identification of such a prognostic marker could potentially affect surgical and adjuvant therapy and/or follow-up surveillance for these patients. Two-dimensional difference gel electrophoresis was utilized to characterize protein expression patterns in lipoma, atypical lipomatous tumor (ALT), and the well-differentiated components of dedifferentiated liposarcoma (DDL). Protein spots were identified by peptide mapping/fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. No significant differences in protein expression were identified between lipoma and ALT or DDL. Proteins that were significantly down-regulated in the well-differentiated component of DDL compared to ALT included mitochondrial aldehyde dehydrogenase 2 (ALDH2, >3-fold reduction) and selenium-binding protein-1 (SELENBP1, >4-fold reduction). Subsequent validation studies were performed by immunohistochemistry (IHC) on a separate series of ALT (n = 30) and the well-differentiated components of DDL (n = 28). IHC stains were evaluated in a semi-quantitative manner, and the results were analyzed using the Mann–Whitney test and receiver–operator curve analysis. Decreased IHC staining for SELENBP1 in the well-differentiated component of DDL was confirmed. Cytoplasmic ALDH2 levels determined by IHC were not significantly different in ALT and DDL; no nuclear staining for ALDH2 was observed. Expression of SELENBP1 is decreased in the well-differentiated component of DDL compared to ALT. However, variability in the staining patterns in liposarcoma precludes its use as a predictive marker for dedifferentiation.

Keywords

LiposarcomaDedifferentiationProteomicsSelenium-binding protein-1Two-dimensional difference gel electrophoresis

Supplementary material

428_2013_1416_MOESM1_ESM.pdf (69 kb)
ESM 1Proteins differentially expressed in lipoma, atypical lipomatous tumor, and dedifferentiated liposarcoma determined by two-dimensional difference gel electrophoresis. (PDF 68 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2013