Virchows Archiv

, Volume 462, Issue 6, pp 639–644

ERG expression and prostatic adenocarcinoma

  • Montse Verdu
  • Isabel Trias
  • Ruth Roman
  • Natalia Rodon
  • Beatriz Garcia-Pelaez
  • Miquel Calvo
  • Arturo Dominguez
  • Josep M. Banus
  • Xavier Puig
Original Article

DOI: 10.1007/s00428-013-1415-3

Cite this article as:
Verdu, M., Trias, I., Roman, R. et al. Virchows Arch (2013) 462: 639. doi:10.1007/s00428-013-1415-3

Abstract

ERG gene rearrangement has been identified as a highly specific alteration that is present in 40–50 % of prostate carcinomas. The standardization of an immunohistochemical assay with a novel anti-ERG antibody recently described would have significant diagnostic value. The aims of this study were to identify the incidence of this rearrangement in a Spanish population and to test the specificity of immunohistochemical ERG evaluation for prostate carcinomas. Three prostate tissue microarrays were constructed using radical prostatectomy specimens and related to grade, local invasion, and regional invasion. In addition to samples from malignant cases (160), specimens of prostatic hyperplasia (26) and high-grade prostatic intraepithelial neoplasia (10) were included. Tissue microarrays of 270 samples from most common malignant tumors (breast, colon, lung, and bladder) were also tested. All were analyzed by immunohistochemistry. Seventy-five out of 154 evaluable cases (49 %) of prostate carcinoma showed ERG expression; 52/75 showed strong staining. No ERG expression was observed in any of the high-grade prostatic intraepithelial neoplasia. ERG expression was independent of Gleason score (p = 0.160), extent of invasion (p = 0.517), and regional lymph node involvement (p = 0.816). No ERG expression was found in any other type of tumor, with the exception of one bladder cancer sample that showed focal and weak expression. The frequency of ERG detected in our study correlated with the results published for other Caucasian populations. Strong ERG protein expression was exclusively detected in prostate carcinomas, corroborating the specificity of ERG rearrangements for these tumors. Thus, detecting ERG using immunohistochemistry may be useful in routine practice in pathology departments.

Keywords

ERG rearrangementProstate cancerImmunohistochemistryClone EPR3864TMPRSS2:ERG

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Montse Verdu
    • 1
    • 2
  • Isabel Trias
    • 1
    • 2
    • 3
    • 6
  • Ruth Roman
    • 1
  • Natalia Rodon
    • 1
  • Beatriz Garcia-Pelaez
    • 1
  • Miquel Calvo
    • 4
  • Arturo Dominguez
    • 5
  • Josep M. Banus
    • 5
  • Xavier Puig
    • 1
    • 2
    • 6
  1. 1.BIOPAT, Biopatologia MolecularGrup AssistenciaBarcelonaSpain
  2. 2.Histopat LaboratorisBarcelonaSpain
  3. 3.Hospital Plato Fundacio PrivadaBarcelonaSpain
  4. 4.Statistics DepartmentUniversitat de Barcelona (UB)BarcelonaSpain
  5. 5.Institut Catala d’Urologia i Nefrologia (ICUN)BarcelonaSpain
  6. 6.Hospital de Barcelona, SCIASGrup AssistenciaBarcelonaSpain