Virchows Archiv

, Volume 461, Issue 5, pp 531–540

Cribriform adenocarcinoma of minor salivary glands may express galectin-3, cytokeratin 19, and HBME-1 and contains polymorphisms of RET and H-RAS proto-oncogenes

  • Jan Laco
  • Kateřina Kamarádová
  • Pavla Vítková
  • Eva Sehnálková
  • Šárka Dvořáková
  • Eliška Václavíková
  • Vlasta Sýkorová
  • Jana Kašpírková
  • Alena Skálová
  • Aleš Ryška
Original Article

DOI: 10.1007/s00428-012-1320-1

Cite this article as:
Laco, J., Kamarádová, K., Vítková, P. et al. Virchows Arch (2012) 461: 531. doi:10.1007/s00428-012-1320-1

Abstract

The aim of the study was to further elucidate the immunohistochemical and genetic characteristics of cribriform adenocarcinoma of minor salivary glands (CAMSG). The study comprised five CAMSG from two males and three females, aged 21–72 years. Four tumors were localized at the base of tongue and one in the floor of mouth. At the time of diagnosis, four tumors had metastasised to regional lymph nodes. After tumor resection, two patients were treated by radiotherapy and one by chemoradiotherapy. During the follow-up (median 14 months), two patients developed lymph node metastasis. Microscopically, all tumors showed cribriform, papillary, follicular, and microcystic growth patterns. The tumor cells displayed vesicular nuclei with intranuclear grooves. Immunohistochemically, all tumors showed expression of cytokeratin (CK) 7, CK8, CK18, vimentin, smooth muscle actin, calponin, S-100 protein, and p16 protein. In addition, we observed expression of galectin-3, CK19, and HBME-1, but not of thyroglobulin and TTF-1. No mutations of RET, BRAF, K-RAS, H-RAS, and N-RAS proto-oncogenes were detected. However, in RET proto-oncogene, we found polymorphisms Gly691Ser (exon 11) and Ser904Ser (exon 15) in one case, p.Leu769Leu (exon 13) in one case, and variant p.IVS14-24 G/A of intron 14 in two cases, and in H-RAS proto-oncogene we found polymorphism 81 T-C (exon 1) in three cases. Thyroglobulin and TTF-1 are the only useful markers in the differential diagnosis between CAMSG and papillary thyroid carcinoma as both tumors may express galectin-3, CK19, and HBME-1. The RET, H-RAS, and N-RAS proto-oncoogenes are not mutated in CAMSG.

Keywords

Salivary glandTongueCribriform adenocarcinomaRET proto-oncogeneH-RAS proto-oncogene

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Jan Laco
    • 1
  • Kateřina Kamarádová
    • 2
  • Pavla Vítková
    • 3
  • Eva Sehnálková
    • 4
  • Šárka Dvořáková
    • 5
  • Eliška Václavíková
    • 5
  • Vlasta Sýkorová
    • 5
  • Jana Kašpírková
    • 6
  • Alena Skálová
    • 6
    • 7
  • Aleš Ryška
    • 1
  1. 1.The Fingerland Department of PathologyCharles University Faculty of Medicine and Faculty HospitalHradec KrálovéCzech Republic
  2. 2.Department of Pathology and Molecular MedicineCharles University 2nd Faculty of Medicine and Faculty Hospital MotolPragueCzech Republic
  3. 3.Department of PathologyHospital České BudějoviceBudweisCzech Republic
  4. 4.Department of PathologySilesian HospitalOpavaCzech Republic
  5. 5.Department of Molecular EndocrinologyInstitute of EndocrinologyPragueCzech Republic
  6. 6.Bioptic LaboratoryPilsenCzech Republic
  7. 7.Department of PathologyCharles University Faculty of MedicinePilsenCzech Republic