Virchows Archiv

, Volume 458, Issue 4, pp 453–465

Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues

Authors

  • Eun Shin
    • Department of PathologySeoul National University College of Medicine
  • Kyoung-Bun Lee
    • Department of PathologySeoul National University College of Medicine
  • Soo-Young Park
    • Department of PathologySeoul National University College of Medicine
  • Soo-Hee Kim
    • Department of PathologySeoul National University College of Medicine
  • Han-Suk Ryu
    • Department of PathologySeoul National University College of Medicine
  • Young-Nyun Park
    • Department of PathologyYonsei University College of Medicine
  • Eunsil Yu
    • Department of PathologySeoul Asan Medical Center
    • Department of PathologySeoul National University College of Medicine
Original Article

DOI: 10.1007/s00428-011-1043-8

Cite this article as:
Shin, E., Lee, K., Park, S. et al. Virchows Arch (2011) 458: 453. doi:10.1007/s00428-011-1043-8

Abstract

We elucidated the genetic profile of hepatoblastomas (HBLs) to identify diagnostic and prognostic markers. RNA was extracted from 32 formalin-fixed, paraffin-embedded HBLs and corresponding nonneoplastic liver (NNL) tissues, and cDNA-mediated annealing, selection, extension, and ligation (DASL) chip assays were performed. Immunohistochemistry was performed to confirm the expression of Yin Yang 1 (YY1) protein in HBL. Twenty-four genes that were associated with signal transduction, cell–cell adhesion, cell cycle regulation, and apoptosis were differentially expressed in HBL and NNL tissues. Two apoptosis-associated genes, MYCN and BIRC5, were highly upregulated in HBL. Eight genes, including YY1 and IGF1, were upregulated in HBL cases that had a poor prognosis. Thirty-eight genes, including YY1, were differentially expressed according to histologic differentiation of HBL, and the immunohistochemical expression of YY1 was correlated with poor HBL differentiation. Thus, using DASL chip assays, we report the gene expression profiles of HBL, which suggest new candidate prognostic and diagnostic genetic markers and putative therapeutic targets for HBL.

Keywords

HepatoblastomaDASL chip assayYY1Prognosis

Copyright information

© Springer-Verlag 2011