Virchows Archiv

, Volume 455, Issue 3, pp 277–284

Loss of RKIP expression is associated with poor survival in GISTs

  • Olga Martinho
  • António Gouveia
  • Paula Silva
  • Amadeu Pimenta
  • Rui Manuel Reis
  • José Manuel Lopes
Original Article

DOI: 10.1007/s00428-009-0821-z

Cite this article as:
Martinho, O., Gouveia, A., Silva, P. et al. Virchows Arch (2009) 455: 277. doi:10.1007/s00428-009-0821-z

Abstract

Gastrointestinal stromal tumours (GISTs) are rare mesenchymal tumours of the digestive tract and are commonly driven by oncogenic mutations in KIT and PDGFRA genes. Tumour size, location, mitotic index and KIT/PDGFRA mutations are the most important prognostic parameters in GISTs. However, additional studies screening for new molecular prognostic markers in GISTs are missing. Raf kinase inhibitor protein (RKIP) has been considered as a suppressor of metastasis and a prognostic marker in several neoplasms. In the present study we aimed to examine whether RKIP expression is associated with GIST clinical–pathological features. Using immunohistochemistry, we determined RKIP expression levels in a well-characterised series of 70 GISTs. We found that RKIP is expressed in the great majority of cases, and absent in approximately 9% of GISTs. Additionally, we found that loss of RKIP expression was not due to the promoter methylation as assessed by methylation-specific PCR. Loss of RKIP expression was associated with poor disease-specific survival and with tumour necrosis in GISTs. Furthermore, a statistical tendency was observed between the positive RKIP expression and absence of metastasis. So far, this is the first study assessing RKIP expression levels in GISTs. We conclude that loss of RKIP expression could have an important role as prognostic marker in GISTs.

Keywords

RKIP Expression Survival GISTs 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Olga Martinho
    • 1
  • António Gouveia
    • 2
    • 3
    • 5
  • Paula Silva
    • 2
    • 5
  • Amadeu Pimenta
    • 2
    • 3
    • 5
  • Rui Manuel Reis
    • 1
  • José Manuel Lopes
    • 2
    • 4
    • 5
  1. 1.Life and Health Sciences Research Institute (ICVS), School of Health SciencesUniversity of MinhoBragaPortugal
  2. 2.IPATIMUPInstitute of Molecular Pathology and Immunology of the University of PortoPortoPortugal
  3. 3.Department of SurgeryPortoPortugal
  4. 4.Department of Pathology, H.S. JoãoPortoPortugal
  5. 5.Medical Faculty of Porto UniversityPortoPortugal

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