Virchows Archiv

, 453:275

Detection of Epstein–Barr virus-encoded small RNA-expressed myofibroblasts and IgG4-producing plasma cells in sclerosing angiomatoid nodular transformation of the spleen

  • Satoko Kashiwagi
  • Toshio Kumasaka
  • Nobukawa Bunsei
  • Yuki Fukumura
  • Shigetaka Yamasaki
  • Keiko Abe
  • Keiko Mitani
  • Hiroshi Abe
  • Toshiharu Matsumoto
  • Koichi Suda
Original Article

DOI: 10.1007/s00428-008-0648-z

Cite this article as:
Kashiwagi, S., Kumasaka, T., Bunsei, N. et al. Virchows Arch (2008) 453: 275. doi:10.1007/s00428-008-0648-z

Abstract

Sclerosing angiomatoid nodular transformation (SANT) of the spleen is a rare inflammatory tumor-like lesion composed of vascular nodules and non-neoplastic stroma including spindle cells and inflammatory cells. The focus of our study was on the stromal proliferating process in SANT. Nine cases of SANT were examined. All cases showed α-smooth muscle actin (α-SMA) and vimentin on the spindle cells but not CD21, CD31, CD34, CD68, desmin, S100, human herpes virus-8, or anaplastic lymphoma kinase-1. In one case, 20–30% of the myofibroblasts in Epstein–Barr-virus (EBV)-positive spindle cells were detected using double-labeling immunohistochemistry for α-SMA and EBV-encoded small RNA in situ hybridization. A quantitative analysis of IgG and IgG4-positive plasma cells (pPCs) in SANT was performed. The median densities of IgG-pPCs and IgG4-pPCs in SANT were approximately four-fold and 13-fold higher than those in the normal spleens, respectively. In addition, there was a statistically significant increase of IgG4/IgG-pPCs ratio in SANT in comparison to the control specimens. In conclusion, the fibrogenesis in a subset of SANT may be associated with EBV-infected myofibroblasts in an overlapping immune reaction indicated by the presence of infiltrating IgG4-pPCs. Further investigation is needed to elucidate the association between SANT and IgG4-related sclerosing disease.

Keywords

Inflammatory pseudotumor Follicular dendritic cell tumor EBER in situ hybridization Bone-marrow-derived stem cells IgG4-related sclerosing disease 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Satoko Kashiwagi
    • 1
  • Toshio Kumasaka
    • 1
  • Nobukawa Bunsei
    • 1
  • Yuki Fukumura
    • 1
  • Shigetaka Yamasaki
    • 2
  • Keiko Abe
    • 3
  • Keiko Mitani
    • 1
  • Hiroshi Abe
    • 1
  • Toshiharu Matsumoto
    • 1
  • Koichi Suda
    • 1
  1. 1.Department of Human PathologyJuntendo University Graduate School of MedicineTokyoJapan
  2. 2.Department of PathologyTokyo Rinkai HospitalTokyoJapan
  3. 3.Department of HistopathologyTohoku University Graduate School of MedicineSendaiJapan