Virchows Archiv

, Volume 452, Issue 3, pp 305–312

A procedure for tissue freezing and processing applicable to both intra-operative frozen section diagnosis and tissue banking in surgical pathology

Authors

  • Susanne Steu
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Maya Baucamp
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Gabriela von Dach
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Marion Bawohl
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Susanne Dettwiler
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Martina Storz
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
  • Holger Moch
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
    • Department of Pathology, Institute of Surgical PathologyZurich University Hospital
Original Article

DOI: 10.1007/s00428-008-0584-y

Cite this article as:
Steu, S., Baucamp, M., von Dach, G. et al. Virchows Arch (2008) 452: 305. doi:10.1007/s00428-008-0584-y

Abstract

Different methods for snap freezing surgical human tissue specimens exist. At pathology institutes with higher work loads, solid carbon dioxide, freezing sprays, and cryostat freezing are commonly used as coolants for diagnosing frozen tissue sections, whereas for tissue banking, liquid nitrogen or isopentane cooled with liquid nitrogen is preferred. Freezing tissues for diagnostic and research purposes are therefore often time consuming, laborious, even hazardous, and not user friendly. In tissue banks, frozen tissue samples are stored in cryovials, capsules, cryomolds, or cryocassettes. Tissues are additionally embedded using freezing media or wrapped in plastic bags or aluminum foils to prevent desiccation. The latter method aggravates enormously further tissue handling and processing. Here, we describe an isopentane-based workflow which concurrently facilitates tissue freezing and processing for both routine intra-operative frozen section and tissue banking and satisfies the qualitative demands of pathologists, cancer researchers, laboratory technicians, and tissue bankers.

Keywords

Rapid frozen sectionTissue bankingTissue freezing proceduresOCT

Copyright information

© Springer-Verlag 2008