Virchows Archiv

, Volume 452, Issue 1, pp 27–32

C-kit protein expression correlated with activating mutations in KIT gene in oral mucosal melanoma

  • Rosario S. Rivera
  • Hitoshi Nagatsuka
  • Mehmet Gunduz
  • Beyhan Cengiz
  • Esra Gunduz
  • Chong Huat Siar
  • Hidetsugu Tsujigiwa
  • Ryo Tamamura
  • Kok Ng Han
  • Noriyuki Nagai
Original Article

DOI: 10.1007/s00428-007-0524-2

Cite this article as:
Rivera, R.S., Nagatsuka, H., Gunduz, M. et al. Virchows Arch (2008) 452: 27. doi:10.1007/s00428-007-0524-2

Abstract

C-kit is a trans-membrane receptor tyrosine kinase (RTK) encoded by the proto-oncogene KIT located at 4q11-12. Gain-of-function mutations arising to c-kit activation independent of its ligand were observed in various tumors related to germ cells, mast cells, and interstitial cells of Cajal. C-kit also participates in melanocyte development; hence, its involvement in oral mucosal melanoma (OMM) tumorigenesis was investigated. Immunohistochemistry and mutation analysis were performed using 18 cases of human primary OMM. Results revealed 16 cases positive to c-kit protein. Atypical melanocytes expressed c-kit. All in situ components expressed c-kit, but only four cases exhibited intense expression in the invasive component. Missense mutations were observed in four cases, and two of those correlated with increased protein expression. C-kit expression in atypical melanocytes suggests the role of c-kit in the early stage of OMM tumorigenesis. C-kit protein expression correlated with activating mutations indicating the pertinent role of the proto-oncogene KIT in the tumorigenesis of OMM.

Keywords

Oral mucosal melanoma C-kit mutation Immunohistochemistry 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Rosario S. Rivera
    • 1
    • 2
  • Hitoshi Nagatsuka
    • 1
  • Mehmet Gunduz
    • 1
  • Beyhan Cengiz
    • 1
  • Esra Gunduz
    • 1
  • Chong Huat Siar
    • 3
  • Hidetsugu Tsujigiwa
    • 1
  • Ryo Tamamura
    • 1
  • Kok Ng Han
    • 4
  • Noriyuki Nagai
    • 1
  1. 1.Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayama UniversityOkayamaJapan
  2. 2.College of DentistryUniversity of the EastManilaPhilippines
  3. 3.Department of Oral Pathology, Oral Medicine and Periodontology, Faculty of DentistryUniversity of MalayaKuala LampurMalaysia
  4. 4.Unit of Stomatology, Cancer Research CentreInstitute for Medical ResearchKuala LumpurMalaysia

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