Virchows Archiv

, Volume 449, Issue 3, pp 308–314

Oncofetal protein glypican-3 in testicular germ-cell tumor

  • Satoshi Ota
  • Michiyo Hishinuma
  • Naoko Yamauchi
  • Akiteru Goto
  • Teppei Morikawa
  • Tetsuya Fujimura
  • Tadaichi Kitamura
  • Tatsuhiko Kodama
  • Hiroyuki Aburatani
  • Masashi Fukayama
Original Article

DOI: 10.1007/s00428-006-0238-x

Cite this article as:
Ota, S., Hishinuma, M., Yamauchi, N. et al. Virchows Arch (2006) 449: 308. doi:10.1007/s00428-006-0238-x

Abstract

The expression of an oncofetal protein, the glypican-3 (GPC3), was immunohistochemically evaluated in a wide variety of primary testicular germ-cell tumors (GCTs) in comparison with other markers, alpha-fetoprotein (AFP), human chorionic gonadotropin (hCG)-beta, and OCT3/4. Eighty-nine cases of GCT including 22 cases of mixed GCT were evaluated with reference to each tumor component. GPC3 expression was observed in neoplastic cells of yolk-sac tumor (YST) (25/25), teratoma (2/10), components of syncytiotrophoblastic giant cells (STGCs) (10/14), and choriocarcinoma (1/3), but none in intratubular germ-cell neoplasias, unclassified type (0/33), seminomas (0/61), or embryonal carcinoma (0/19). All cases of YST showed diffuse labeling of neoplastic cells in cytoplasmic and membranous patterns, and the positive area of GPC3 was much larger than that of AFP. Glandular structures in teratomas showed GPC3 immunostaining as well as AFP. Although the number of GPC3-positive cells was smaller in STGC components and choriocarcinoma, there was no diffusion artifact in GPC3 immunostaining, as was frequently encountered in hCG-beta staining. Thus, GPC3 is a unique oncofetal protein, which is useful as an immunohistochemical marker for GCT differentiated to extraembryonic tissue, especially YST.

Keywords

TestisGlypican-3Germ-cell tumorNeoplasiaYolk-sac tumorBiomarker

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Satoshi Ota
    • 1
  • Michiyo Hishinuma
    • 1
  • Naoko Yamauchi
    • 1
  • Akiteru Goto
    • 1
  • Teppei Morikawa
    • 1
  • Tetsuya Fujimura
    • 2
  • Tadaichi Kitamura
    • 2
  • Tatsuhiko Kodama
    • 3
  • Hiroyuki Aburatani
    • 3
  • Masashi Fukayama
    • 1
  1. 1.Department of Pathology, Graduate School of MedicineUniversity of TokyoTokyoJapan
  2. 2.Department of Urology, Graduate School of MedicineUniversity of TokyoTokyoJapan
  3. 3.Genome Science Division, Research Center for Advanced Science and TechnologyUniversity of TokyoTokyoJapan