Virchows Archiv

, 449:200

Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours

  • Si Brask Sonne
  • Amy S. Herlihy
  • Christina E. Hoei-Hansen
  • John E. Nielsen
  • Kristian Almstrup
  • Niels E. Skakkebaek
  • Alexander Marks
  • Henrik Leffers
  • Ewa Rajpert-De Meyts
Original Article

DOI: 10.1007/s00428-006-0223-4

Cite this article as:
Sonne, S.B., Herlihy, A.S., Hoei-Hansen, C.E. et al. Virchows Arch (2006) 449: 200. doi:10.1007/s00428-006-0223-4

Abstract

Testicular germ-cell tumours of young adults are derived from a pre-invasive intratubular lesion, carcinoma in situ (CIS). In a recent genome-wide gene expression screening using cDNA microarrays, we found PDPN over-expressed in CIS compared to normal adult testis. PDPN encodes podoplanin (Aggrus, human gp36, T1A-2), a transmembrane glycoprotein expressed in lymphatic endothelium and various solid tumours. To examine a potential role for PDPN in testicular neoplasms and during testicular development, we investigated its expression pattern during the development of human testis and in a series of testicular CIS, gonadoblastoma and overt germ-cell tumours. We established by RT-PCR and by immunohistochemistry with a gp36 antibody that PDPN mRNA and the protein product were expressed in testes with germ-cell neoplasms but not in the normal adult testis. We also found gp36 expression in early foetal gonocytes and immature Sertoli cells, similar to the expression pattern of M2A antigen, a previously identified marker for CIS and seminoma. This reinforced our previous proposal that M2A (D2-40) antigen was identical to gp36 (podoplanin, Aggrus, T1A-2). Our findings also suggest that podoplanin has a function in developing testis, most likely at the level of cell–cell interactions among pre-meiotic germ cells and immature Sertoli cells.

Keywords

M2A PDPN Intratubular germ-cell neoplasia (IGCN) Testicular germ-cell neoplasm Testicular development Sertoli cells 

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Si Brask Sonne
    • 1
  • Amy S. Herlihy
    • 1
  • Christina E. Hoei-Hansen
    • 1
  • John E. Nielsen
    • 1
  • Kristian Almstrup
    • 3
  • Niels E. Skakkebaek
    • 1
  • Alexander Marks
    • 2
  • Henrik Leffers
    • 1
  • Ewa Rajpert-De Meyts
    • 1
  1. 1.University Department of Growth and ReproductionCopenhagenDenmark
  2. 2.Banting and Best Department of Medical ResearchUniversity of TorontoTorontoCanada
  3. 3.Novo NordiskMolecular GeneticsBagsvaerdDenmark

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