Virchows Archiv

, Volume 446, Issue 4, pp 438–441

Evidence of a polyclonal nature of myositis ossificans

Authors

  • Andreas Leithner
    • Department of Orthopedic SurgeryMedical University Graz
  • Andreas Weinhaeusel
    • Children’s Cancer Research Institute (CCRI)St. Anna Children’s Hospital
  • Petra Zeitlhofer
    • Children’s Cancer Research Institute (CCRI)St. Anna Children’s Hospital
  • Horst Koch
    • Department of Surgery, Division of Plastic SurgeryMedical University Graz
  • Roman Radl
    • Department of Orthopedic SurgeryMedical University Graz
  • Reinhard Windhager
    • Department of Orthopedic SurgeryMedical University Graz
  • Alfred Beham
    • Institute of PathologyMedical University Graz
    • Children’s Cancer Research Institute (CCRI)St. Anna Children’s Hospital
Original Article

DOI: 10.1007/s00428-004-1169-z

Cite this article as:
Leithner, A., Weinhaeusel, A., Zeitlhofer, P. et al. Virchows Arch (2005) 446: 438. doi:10.1007/s00428-004-1169-z

Abstract

Myositis ossificans is a localized, self-limiting, reparative lesion that is composed of reactive hypercellular fibrous tissue and bone. Although it is clearly a benign lesion, its clinical, radiological, and histological appearance may sometimes mimic a malignant tumor. Whether myositis ossificans represents a monoconal or polyclonal hyperplastic proliferation is not yet known. To address this question, we therefore extracted DNA from the respective paraffin-embedded tumor tissues of nine women with a median age of 50 years at diagnosis (range: 20–84 years) and studied the X inactivation pattern by means of methylation-sensitive polymerase chain reaction and primers that target the polymorphic CGG trinucleotide repeat of the FMR1 gene. The fact that we did not detect any skewing of the X inactivation pattern in the five successfully analyzed cases corroborates the notion that myositis ossificans results from a polyclonal proliferation and confirms that it is a reactive, reparative process. Analysis of the X inactivation pattern may, thus, supplement the differential diagnostic work-up of cases with an uncertain histology, at least in the informative proportion of female patients.

Keywords

Myositis ossificansDNA methylationX inactivationPolymerase chain reactionFMR1

Copyright information

© Springer-Verlag 2005