Virchows Archiv

, Volume 446, Issue 1, pp 15–20

In angioimmunoblastic T-cell lymphoma, neoplastic T cells may be a minor cell population. A molecular single-cell and immunohistochemical study

  • Klaus Willenbrock
  • Christoph Renné
  • Philippe Gaulard
  • Martin-Leo Hansmann
Original Article

DOI: 10.1007/s00428-004-1114-1

Cite this article as:
Willenbrock, K., Renné, C., Gaulard, P. et al. Virchows Arch (2005) 446: 15. doi:10.1007/s00428-004-1114-1

Abstract

The significance of T-cell proliferations in angioimmunoblastic lymphoma (AILD) is still enigmatic. Although classified as a malignant T-cell lymphoma in the World Health Organisation lymphoma classification, some cases of AILD lack dominant T-cell clones. In a previous study, based on single-cell polymerase chain reaction (PCR), we obtained similar results as studies of AILD using Southern blot or conventional PCR: some cases of AILD contained large T-cell clones, and, in other cases, T-cell clones were undetectable. As in single-cell studies, only a limited number of cells could be investigated; thus, we wanted to gain more insight into the amount and distribution of tumour cells. By applying triple immunofluorescent staining with antibodies directed against T-cell receptor Vβ-family-specific epitopes, we investigated T-cell populations in AILD and their localisation in the tissue in relation to B cells (CD20) and follicular dendritic cells (CD21). In two of five cases investigated, only a minority of the T-cells compartment belonged to the tumour clone. Neoplastic T cells were found throughout the tissue, including areas dominated by B cells.

Keywords

AILD T-cells B-cells Clonality Single-cell PCR 

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Klaus Willenbrock
    • 1
  • Christoph Renné
    • 1
  • Philippe Gaulard
    • 2
  • Martin-Leo Hansmann
    • 1
  1. 1.Senckenbergisches Institut für PathologieKlinikum der Johann Wolfgang Goethe UniversitätFrankfurt am MainGermany
  2. 2.Department of PathologyHôpital Henri MondorCréteilFrance