Virchows Archiv

, Volume 441, Issue 6, pp 605–613

CD38 is a marker of human lacteals

Authors

  • Inger Farstad
    • Laboratory for Immunohistochemistry and Immunopathology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway
  • Fabio Malavasi
    • Laboratory of Transplant Immunology, Department of Genetics, Biology and Biochemistry, University of Torino, Turin, Italy
  • Guttorm Haraldsen
    • Laboratory for Immunohistochemistry and Immunopathology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway
  • Henrik Huitfeldt
    • Section for Toxicopathology, Institute of Pathology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway
  • Per Brandtzaeg
    • Laboratory for Immunohistochemistry and Immunopathology, Rikshospitalet, University of Oslo, 0027 Oslo, Norway
Original Article

DOI: 10.1007/s00428-002-0679-9

Cite this article as:
Farstad, I., Malavasi, F., Haraldsen, G. et al. Virchows Arch (2002) 441: 605. doi:10.1007/s00428-002-0679-9
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Abstract

Abstract. CD38 is a type II transmembrane glycoprotein involved in signaling and adhesion which is expressed mainly by immature hematopoietic cells and activated lymphoid cells. Central lymphatic channels of human small intestinal villi, the so-called lacteals, were coincidentally found to express CD38. Gastric and large intestinal mucosae, pancreas, liver, lung, nasal mucosa, kidney, thymus, palatine tonsil, Peyer's patches, appendix, and mesenteric lymph nodes, and rodent intestinal mucosa were subsequently examined for lymphatic expression of CD38. Cryosections prepared from biopsy or surgical resection specimens were immunostained with four different antibodies to CD38 combined with antibodies to von Willebrand factor and CD31 to differentiate lymphatics from blood vessels, or with antibody to lysosomal protein. Sections were evaluated by ordinary and confocal immunofluorescence microscopy. Jejunal cryosections were subjected to in situ hybridization for CD38. All CD38 antibodies decorated human lacteals, and some of these were positive for CD38 mRNA. Lymphatics draining Peyer's patches and appendix as well as afferent lymphatics of mesenteric lymph nodes expressed CD38 weakly. CD38 was not detected on lymphatics in other organs or in rodent lacteals. We propose that CD38 is a novel marker of human small intestinal lymphatic vessels.

CD38 Lymphatic vessels Humans Rodents
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© Springer-Verlag 2002