Development Genes and Evolution

, Volume 207, Issue 5, pp 362–367

Automated in situ detection (AISD) of biomolecules

  • G. Plickert
  • Martin Gajewski
  • Gerd Gehrke
  • Heinrich Gausepohl
  • Jörg Schlossherr
  • Hady Ibrahim
TECHNICAL NOTE

DOI: 10.1007/s004270050124

Cite this article as:
Plickert, G., Gajewski, M., Gehrke, G. et al. Dev Gene Evol (1997) 207: 362. doi:10.1007/s004270050124

Abstract

 In order to facilitate in situ detection of biomolecules in large sample series the processing of whole-mount specimens has been automated. A freely programmable liquid handling system is described by which embryos or similar biological materials are processed. Possible applications include in situ hybridization (ISH), immunocytochemistry (ICC) or reporter gene assays. Process times required for the preparation of whole-mount in situ hybridizations in Drosophila, Xenopus, Gallus and in hydroids were – in part – significantly reduced as compared with manual processing. Application of automated in situ detection (AISD) in random screening is demonstrated in hydroids. Potential further applications are discussed.

Key words Hydractinia echinataWhole-mount in situ hybridizationExpression analysisRandom screenDifferential display

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • G. Plickert
    • 1
  • Martin Gajewski
    • 1
  • Gerd Gehrke
    • 1
  • Heinrich Gausepohl
    • 2
  • Jörg Schlossherr
    • 1
  • Hady Ibrahim
    • 1
  1. 1.Zoologisches Institut, Universität zu Köln, Weyertal 119, D-50923 Köln, GermanyDE
  2. 2.Abimed-Analysentechnik, Raiffeisenstrasse 3, D-40764 Langenfeld, GermanyDE