Development Genes and Evolution

, Volume 207, Issue 2, pp 71–81

A clonal analysis of spinal cord development in the zebrafish

  • Cyrus Papan
  • José A. Campos-Ortega
ORIGINAL ARTICLE

DOI: 10.1007/s004270050093

Cite this article as:
Papan, C. & Campos-Ortega, J. Dev Gene Evol (1997) 207: 71. doi:10.1007/s004270050093

Abstract

 We describe the results of a clonal analysis of spinal cord development in the zebrafish. The data were obtained from embryos in which fluorescent lineage tracer was injected into single cells in the neural plate at the two-somite stage. Injected animals were allowed to survive until either 4 days or 2 weeks postfertilization. In other experiments, bromodeoxy uridine (BrdU) was injected intraperitoneally at 30 h postfertilization (hpf) after lineage tracer injection in the neural plate at the two-somite stage, and the embryos fixed at 38 hpf. We restricted our experiments to the thoracic region of the spinal cord. Our experiments were aimed at answering questions regarding the proliferative abilities of neuroepithelial cells during embryonic development (as deduced from the size of the clones), the modes of cell division (as deduced from the uptake of BrdU into clone cells), positional differences in the proliferation of cells within the neural plate itself, the cellular composition of the clones, and cell dispersion (deduced from the regional distribution of clone cells).

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Cyrus Papan
    • 1
  • José A. Campos-Ortega
    • 1
  1. 1.Institut für Entwicklungsbiologie, Universität zu Köln, 50923 Köln, GermanyDE