Development Genes and Evolution

, Volume 210, Issue 12, pp 623–629

Highly sensitive, fluorescent transformation marker for Drosophila transgenesis

  • Carsten Horn
  • Brigitte Jaunich
  • E. A. Wimmer
Short Communication

DOI: 10.1007/s004270000111

Cite this article as:
Horn, C., Jaunich, B. & Wimmer, E. Dev Gene Evol (2000) 210: 623. doi:10.1007/s004270000111

Abstract 

The efficiency of transposon-mediated germline transformation is dependent on the transposon mobility in the host embryo, and on the detectability of the used transformation marker. Therefore, high susceptibility of the transformation marker to position effect suppression is a disadvantage. Here we present data that the eye-specific expression of green fluorescent protein, driven by the 3xP3-EGFP marker, outperforms the commonly used ”mini”-white transformation marker in Drosophila germline transformation experiments: 3xP3-EGFP is more sensitive than ”mini”-white in identifying transgenic individuals and reacts differently to position effect suppression. Therefore, 3xP3-EGFP offers an ideal marker for applications in functional genomics where as many gene loci as possible should be targeted in the genome of a specific organism, for example, as intended in the Drosophila gene disruption project. Furthermore, we give a detailed description of the embryonic and larval expression mediated by the 3xP3-EGFP marker. These pre-adult expression patterns, and the potentially universal applicability of the transformation marker also offer additional advantages for selecting transgenic individuals in organisms other than Drosophila. This will be of great interest to the field of evolutionary developmental biology and to modern pest management programs.

Keywords Germline transformationGreen fluorescent proteinPax-6Transposable elementsTransposon mutagenesis

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Carsten Horn
    • 1
  • Brigitte Jaunich
    • 1
  • E. A. Wimmer
    • 1
  1. 1.Lehrstuhl für Genetik, Universität Bayreuth, Universitätsstrasse 30 NWI, 95447 Bayreuth, Germany e-mail: ernst.wimmer@uni-bayreuth.de Tel.: +49-921-555813, Fax: +49-921-552710DE