Original Article

Development Genes and Evolution

, Volume 218, Issue 5, pp 237-251

Expression patterns of engrailed and dpp in the gastropod Lymnaea stagnalis

  • Minoru IijimaAffiliated withDepartment of Earth Evolution Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • , Takeshi TakeuchiAffiliated withDepartment of Earth Evolution Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • , Isao SarashinaAffiliated withDepartment of Earth Evolution Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba
  • , Kazuyoshi EndoAffiliated withDepartment of Earth Evolution Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba Email author 

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Abstract

We isolated the full-length cDNAs of engrailed and dpp-BMP2/4 orthologues from the pond snail Lymnaea stagnalis and examined their expression patterns during development by the whole mount in situ hybridization. At the gastrula and trochophore stages, engrailed is expressed in the peripheral ectoderm of the presumptive and invaginating shell gland, corroborating its role in the shell formation that is widely conserved among molluscs. At the same stages, dpp-BMP2/4 is expressed in the right-hand side ectoderm of the shell gland and in the invaginating stomodaeum. Unlike in the gastropod Patella vulgata, our results suggested that dpp-BMP2/4 has a role in the shell formation, rather than in the regional specification and that it could be involved in the specification pathway of the left–right asymmetry of the developing shell in L. stagnalis.

Keywords

Gastropod mollusk Lymnaea stagnalis Shell formation Engrailed dpp-BMP2/4