Development Genes and Evolution

, Volume 215, Issue 8, pp 393–401

Afuni, a novel transforming growth factor-β gene is involved in arm regeneration by the brittle star Amphiura filiformis

  • R. Bannister
  • I. M. McGonnell
  • A. Graham
  • M. C. Thorndyke
  • P. W. Beesley
Original Article

DOI: 10.1007/s00427-005-0487-8

Cite this article as:
Bannister, R., McGonnell, I.M., Graham, A. et al. Dev Genes Evol (2005) 215: 393. doi:10.1007/s00427-005-0487-8

Abstract

The bone morphogenetic proteins (BMPs) are a family of the transforming growth factor-β (TGF-β) superfamily that perform multiple roles during vertebrate and invertebrate development. Here, we report the molecular cloning of a novel BMP from regenerating arms of the ophiuroid Amphiura filiformis. The theoretically translated amino acid sequence of this novel BMP has high similarity to that of the sea urchin BMP univin. This novel BMP has been named afuni. Whole-mount in situ hybridisation implicates afuni in arm regeneration. Expression occurs in distinct proximal and distal regions of late regenerates (3- and 5-week postablation). These sites are at different stages of regeneration, suggesting multiple roles for this gene in adult arm development. Cellular expression of this gene occurs in migratory cells within the radial water canal (RWC) of regenerating and nonregenerating arms. These migrating coelomocytes suggest a key role for the coelomic RWC as a source of the cellular material for use in arm regeneration by A. filiformis.

Keywords

Amphiura filiformisBone morphogenetic proteinCoelomic canalRegeneration

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • R. Bannister
    • 1
    • 5
  • I. M. McGonnell
    • 2
  • A. Graham
    • 3
  • M. C. Thorndyke
    • 4
  • P. W. Beesley
    • 1
  1. 1.School of Biological Sciences, Royal HollowayUniversity of LondonSurreyUK
  2. 2.Department of Veterinary Basic SciencesRoyal Veterinary CollegeLondonUK
  3. 3.MRC Centre for Developmental NeurobiologyKings College LondonLondonUK
  4. 4.Royal Swedish Academy of SciencesKristineberg Marine Research StationFiskebackskilSweden
  5. 5.Institute for the EnvironmentBrunel UniversityMiddlesexUK