Development Genes and Evolution

, Volume 215, Issue 5, pp 238–247

Differential expression of caudal and dorsal genes in the teloblast lineages of the oligochaete annelid Tubifex tubifex

Original Article

DOI: 10.1007/s00427-005-0473-1

Cite this article as:
Matsuo, K., Yoshida, H. & Shimizu, T. Dev Genes Evol (2005) 215: 238. doi:10.1007/s00427-005-0473-1


We have cloned homologues (designated Ttu-cdx and Ttu-dl) of caudal and dorsal genes from the oligochaete annelid Tubifex tubifex. In situ hybridization revealed that Ttu-cdx begins to be expressed around the time of completion of ectodermal teloblastogenesis (i.e., the onset of gastrulation). At this time, Ttu-cdx expression is detected in a few cells that are located in the anteriormost part of the mesodermal germ bands (GBs). As development proceeds and the GBs elongate, the domain of Ttu-cdx-expressing cells in the GBs extends posteriorly. During this process, M teloblasts and primary blast cells remained negative for Ttu-cdx expression. This Ttu-cdx expression pattern is in sharp contrast to the posterior caudal expression in other organisms. Expression of Ttu-dl is detected from the one-cell stage through to the gastrula stage. Although Ttu-dl is expressed in most blastomeres, there are regional differences in Ttu-dl expression levels. During early cleavage, a large amount of Ttu-dl mRNA, which is supplied maternally, is inherited by D-cell line micromeres 2d and 4d; the remaining micromeres and macromeres inherit relatively small amounts of Ttu-dl transcripts. Another regional difference in Ttu-dl expression levels is seen in the ectodermal GB that is comprised of four bast-cell bandlets. The ventralmost bandlet (N lineage) exhibits the highest level of Ttu-dl expression with the lowest level in the two middle bandlets (O and P lineages) and an intermediate level in the dorsalmost bandlet (Q lineage).


Caudal genes Dorsal genes Dorsoventral polarity Teloblast lineages Tubifex 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Division of Biological Sciences, Graduate School of Science Hokkaido UniversitySapporoJapan

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