Development Genes and Evolution

, Volume 213, Issue 9, pp 456–463

The expression of gelatinase A (MMP-2) is required for normal development of zebrafish embryos

  • Jinsong Zhang
  • Shan Bai
  • Xiaoming Zhang
  • Hideaki Nagase
  • Michael P. SarrasJr.
Original Article

DOI: 10.1007/s00427-003-0346-4

Cite this article as:
Zhang, J., Bai, S., Zhang, X. et al. Dev Genes Evol (2003) 213: 456. doi:10.1007/s00427-003-0346-4
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Abstract

Gelatinase A, also called matrix metalloproteinase 2 (MMP-2), belongs to the matrix metalloproteinase (MMP) family. MMP-2 cleaves type IV collagen, denatured collagen (gelatin), and other extracellular matrix (ECM) components. MMP-2 has been reported to be involved in a number of biological and pathological processes, but previous studies have not indicated that its expression is essential for early embryogenesis. In the current study, we have utilized zebrafish as a developmental model to study the role of MMP-2 during embryogenesis. We have successfully isolated a zebrafish MMP-2 (zMMP-2) homologue showing over 80% identity and over 90% similarity to its human counterpart. In situ analysis showed that zMMP-2 was expressed as early as the one-cell stage implying a maternal origin during oogenesis, and embryos continued to express zMMP-2 through at least the 72-h stage of development. RT-PCR analysis confirmed the in situ expression pattern and gelatin zymography indicated that a metalloproteinase with the same gel mobility as vertebrate MMP-2 was present in zebrafish embryos. Injection of zMMP-2 antisense morpholino oligonucleotides into 1- to 4-cell embryos resulted in a truncated axis, monitored through 72 h of development indicating that this metalloproteinase plays an important role in zebrafish embryogenesis. Monpholino-induced alterations in development began to be observed at 12 h of embryogenesis based on morphological and axis marker studies. The results obtained in zebrafish are in contrast to murine knockout studies that indicate that MMP-2 does not have a major role in mouse embryogenesis.

Keywords

Matrix metalloproteinases MMP-2 Zebrafish Development ECM 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Jinsong Zhang
    • 1
    • 2
  • Shan Bai
    • 1
  • Xiaoming Zhang
    • 1
  • Hideaki Nagase
    • 1
    • 3
  • Michael P. SarrasJr.
    • 1
  1. 1.Department of Anatomy and Cell BiologyUniversity of Kansas Medical CenterKansas CityUSA
  2. 2.Department of Pharmaceutical ChemistryUniversity of KansasLawrenceUSA
  3. 3.Kennedy Institute of Rheumatology/Division, Faculty of MedicineImperial College of Science Technology and MedicineLondonUK

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