Development Genes and Evolution

, Volume 213, Issue 7, pp 345–354

Analysis of the biological functions of a doublesex homologue in Bombyx mori

Authors

  • Masataka G. Suzuki
    • Laboratory of Molecular Entomology and BaculovirologyThe Institute of Physical and Chemical Research (RIKEN)
  • Shunsuke Funaguma
    • Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life SciencesThe University of Tokyo
  • Toshio Kanda
    • Insect Gene Engineering LaboratoryNational Institute of Agrobiological Science
  • Toshiki Tamura
    • Insect Gene Engineering LaboratoryNational Institute of Agrobiological Science
    • Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life SciencesThe University of Tokyo
Original Article

DOI: 10.1007/s00427-003-0334-8

Cite this article as:
Suzuki, M.G., Funaguma, S., Kanda, T. et al. Dev Genes Evol (2003) 213: 345. doi:10.1007/s00427-003-0334-8

Abstract

We have previously reported that Bmdsx, a homologue of the sex-determining gene doublesex (dsx), was sex-specifically expressed in various tissues of the silkworm. The primary transcript of Bmdsx is alternatively spliced in males and females to yield sex-specific mRNAs that encode male-specific (BmDSXM) and female-specific (BmDSXF) polypeptides. In the studies reported here, we expressed BmDSXF in males from a ubiquitous promoter and examined its regulatory activities. We show that BmDSXF functions as a positive regulator of the hexameric storage protein termed SP1 and vitellogenin genes that are predominantly expressed in females. We also show that expression of BmdsxF in males results in the repression of the pheromone-binding protein gene that is preferentially expressed in males. Gel-mobility shift assays demonstrated that BmDSX proteins bind to the sequence (ACATTGT) between −95 and −89 nt relative to the transcriptional initiation site of the vitellogenin gene. These results strongly suggest that Bmdsx is a final regulatory gene in the hierarchy of regulatory genes controlling the expression of female-specific protein in Bombyx mori.

Keywords

Bombyx moriSex determinationdoublesexGermline transformationVitellogenin

Copyright information

© Springer-Verlag 2003