Development Genes and Evolution

, Volume 212, Issue 2, pp 70–80

HSP90 function is required for morphogenesis in ascidian and echinoid embryos

Authors

  • Cory D. Bishop
    • Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada
  • William R. Bates
    • Department of Biology, NK Campus, Okanagan University College, 3333 College Way, Kelowna, BC, V1V 1V7, Canada
  • Bruce P. Brandhorst
    • Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada
Original Article

DOI: 10.1007/s00427-002-0212-9

Cite this article as:
Bishop, C.D., Bates, W.R. & Brandhorst, B.P. Dev Genes Evol (2002) 212: 70. doi:10.1007/s00427-002-0212-9

Abstract.

Treatment of embryos of the ascidians Boltenia villosa and Cnemidocarpa finmarkiensis and the sea urchin Strongylocentrotus purpuratus with the anti-HSP90 drugs geldanamycin and radicicol caused morphogenetic arrest. All embryonic stages during which obvious morphogenesis was observed were sensitive to treatment, including formation of the sea urchin blastular epithelium. Arrested embryos were viable for many hours to days post-treatment, indicating a low general toxicity of these drugs. Morphogenetic movements including gastrulation and migration (but not ingression) of sea urchin primary and secondary mesenchyme cells were arrested 8–10 h after treatment began. Cell division and developmentally regulated expression of some genes continued after morphogenesis was arrested. Anti-HSP90 drugs cause selective inactivation or degradation of proteins with which the protein chaperone HSP90 interacts. Therefore, morphogenetic arrest subsequent to the disruption of HSP90 function may result from the reduction in concentration, or activity, of client proteins required for morphogenetic movements of cells. The use of these drugs may provide a means to identify novel activities or proteins involved in morphogenesis.

Molecular chaperone Gastrulation Mesenchyme Epithelium
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Copyright information

© Springer-Verlag 2002