Planta

, Volume 214, Issue 2, pp 235–242

A (1→4)-β-mannan-specific monoclonal antibody and its use in the immunocytochemical location of galactomannans

  • Filomena A. Pettolino
  • Nicholas J. Hoogenraad
  • Charles Ferguson
  • Antony Bacic
  • Elizabeth Johnson
  • Bruce A. Stone
Original Article

DOI: 10.1007/s004250100606

Cite this article as:
Pettolino, F.A., Hoogenraad, N.J., Ferguson, C. et al. Planta (2001) 214: 235. doi:10.1007/s004250100606

Abstract.

Galactomannan was coupled to a protein carrier for the preparation of monoclonal antibodies. The monoclonal antibodies generated bound to galactomannans from different sources as well as to glucomannan and galactoglucomannan. One monoclonal antibody, BGM C6, was characterised and found to be specific for (1→4)-β-linked mannopyranosyl residues; it had a binding affinity estimated at 1×10–6 M for the (1→4)-β-linked mannohexaose. BGM C6 was used in immunogold labelling studies to locate galactomannans in the endosperm walls of normal coconuts (Cocos nucifera L.) and those of the mutant makapuno at two different developmental stages. The pattern and intensity of antibody labelling varied for each type of coconut at the mature and immature stages, indicating differences in the galactomannan composition of the endosperm walls.

Cocos nucifera (endosperm walls) Endosperm (immunocytochemistry) Galactomannan (1→4)-β-Mannan Monoclonal antibody BGM C6

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Filomena A. Pettolino
    • 1
  • Nicholas J. Hoogenraad
    • 1
  • Charles Ferguson
    • 2
  • Antony Bacic
    • 2
  • Elizabeth Johnson
    • 1
  • Bruce A. Stone
    • 1
  1. 1.Department of Biochemistry, La Trobe University, Victoria 3083, Australia
  2. 2.CRC for Bioproducts, Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Victoria 3010, Australia
  3. 3.Present address: CRC for Bioproducts, Plant Cell Biology Research Centre, School of Botany, The University of Melbourne, Victoria 3010, Australia