Planta

, Volume 213, Issue 2, pp 323–326

Biosynthesis of 2-methyl-3-buten-2-ol emitted from needles of Pinus ponderosa via the non-mevalonate DOXP/MEP pathway of isoprenoid formation

Authors

  • Johannes Zeidler
    • Botanisches Institut II der Universität Karlsruhe, Kaiserstrasse 12, 76128 Karlsruhe, Germany
  • Hartmut K. Lichtenthaler
    • Botanisches Institut II der Universität Karlsruhe, Kaiserstrasse 12, 76128 Karlsruhe, Germany
Rapid Communication

DOI: 10.1007/s004250100562

Cite this article as:
Zeidler, J. & Lichtenthaler, H.K. Planta (2001) 213: 323. doi:10.1007/s004250100562

Abstract.

The volatile hemiterpene 2-methyl-3-buten-2-ol (MBO) is emitted from the needles of several pine species from the Western United States and contributes to ozone formation in the atmosphere. It is synthesised enzymatically from dimethylallyl diphosphate (DMAPP). We show here that needles of Pinus ponderosa Laws. incorporated [1-2H1]-1-deoxy-D-xylulose (d-DOX) into the emitted MBO, but not D,L-[2-13C]mevalonic acid lactone. Furthermore, MBO emission was inhibited by fosmidomycin, a specific inhibitor of the second enzyme of the mevalonate-independent pathway of isopentenyl diphosphate and DMAPP formation, i.e. the 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. We thus prove that MBO emitted from needles of P. ponderosa is primarily formed via the DOXP/MEP pathway.

1-Deoxy-D-xylulose Emission (volatile hemiterpene) Fosmidomycin 2-Methyl-3-buten-2-ol Mevalonate Pinus (2-methyl-3-buten-2-ol emission)
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© Springer-Verlag 2001