Planta

, Volume 210, Issue 1, pp 80–84

Osmotic water permeability of isolated vacuoles

Authors

  • Raphaël Morillon
    • UPRESA CNRS 6037, Université de Rouen, Faculté des Sciences, F-76821 Mont-Saint-Aignan cedex, France
  • Jean-Paul Lassalles
    • UPRESA CNRS 6037, Université de Rouen, Faculté des Sciences, F-76821 Mont-Saint-Aignan cedex, France
Article

DOI: 10.1007/s004250050656

Cite this article as:
Morillon, R. & Lassalles, J. Planta (1999) 210: 80. doi:10.1007/s004250050656

Abstract.

We measured the osmotic water permeability (Pos) of vacuoles isolated from onion (Allium cepa L.), rape (Brassica napus L.), petunia (Petunia hybrida Hook.) and red beet (Beta vulgaris L.). For all the vacuolar types investigated, Pos values were in the range 200–1000 μm s−1. The change in membrane surface area induced by an osmotic gradient was smaller than 2–6%. The vacuolar Pos values for red beet and onion were reduced by 1 mM HgCl2, to 14% and 30% of the control values, respectively, but were partially restored to 51% and 76% by 5 mM β-mercaptoethanol. These results suggest that aquaporins were present in all the vacuoles tested. In HgCl2-treated onion vacuoles, the reduced Pos (56 μm s−1) had a low activation energy (approx. 6 kJ mol−1), indicating that water permeation was still occurring mainly via aquaporins, and that the water permeability of the lipid part of the vacuolar membrane is probably very low.

Key words: AlliumBrassicaHgCl2Osmotic water permeabilityVacuole

Copyright information

© Springer-Verlag Berlin Heidelberg 1999