Planta

, Volume 209, Issue 1, pp 13–24

Isolation and expression analysis of two stress-responsive sucrose-synthase genes from the resurrection plant Craterostigma plantagineum (Hochst.)

  • Michael Kleines
  • Ralph-Cyrus Elster
  • Maria-Jesus Rodrigo
  • Anne-Sophie Blervacq
  • Francesco Salamini
  • Dorothea Bartels
Article

DOI: 10.1007/s004250050602

Cite this article as:
Kleines, M., Elster, RC., Rodrigo, MJ. et al. Planta (1999) 209: 13. doi:10.1007/s004250050602

Abstract.

The enzyme sucrose synthase (UDP-glucose: D-fructose 2α-glucosyltransferase, EC 2.4.1.13) is a key enzyme in carbohydrate metabolism, catalyzing the reversible conversion of sucrose uridine-diphosphate into fructose and UDP-glucose. We report the molecular characterization of two classes of cDNA and genomic clones encoding sucrose synthase from Craterostigma plantagineum Hochst., a resurrection plant in which the turnover of sucrose is considered to have an important role in the unique phenomenon of surviving desiccation. Sucrose-synthase transcript and protein levels are modulated by dehydration and rehydration. In-situ hybridization revealed that transcripts preferentially accumulate in phloem tissues. Promoter analysis underlined a role for class-I sucrose-synthase genes in dehydration stress and in response to cis-abscisic acid. A DNA sequence motif common to class-I sucrose-synthase and sucrose-phosphate-synthase genes was discovered.

Key words:Craterostigma Dehydration stress Gene isolation Promoter analysis Resurrection plant Sucrose synthase 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Michael Kleines
    • 1
  • Ralph-Cyrus Elster
    • 1
  • Maria-Jesus Rodrigo
    • 1
  • Anne-Sophie Blervacq
    • 1
  • Francesco Salamini
    • 1
  • Dorothea Bartels
    • 1
  1. 1.Max Planck Institut für Züchtungsforschung, Carl von Linné Weg 10, D-50829 Köln, GermanyDE

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