Planta

, Volume 208, Issue 1, pp 12–18

Maize glutathione-dependent formaldehyde dehydrogenase: protein sequence and catalytic properties

  • Ulrike Wippermann
  • Judith Fliegmann
  • Guy Bauw
  • Christian Langebartels
  • Konrad Maier
  • Heinrich Sandermann
Article

DOI: 10.1007/s004250050529

Cite this article as:
Wippermann, U., Fliegmann, J., Bauw, G. et al. Planta (1999) 208: 12. doi:10.1007/s004250050529

Abstract.

Glutathione-dependent formaldehyde dehydrogenase (FDH; EC 1.2.1.1) has been purified 3900-fold from maize cell-suspension cultures to a specific activity of 4.68 μmol (mg protein)−1 min−1. The homogeneous enzyme consisted of two identical subunits with a molecular mass of 42 kDa, and an isoelectric point of 5.8. Eight tryptic peptides were sequenced and gave a perfect fit to the protein sequence derived from maize Fdh cDNA (J. Fliegmann and H. Sandermann, 1997, Plant Mol Biol 34: 843–854). There was 62% identity with the eucaryotic FDH consensus sequence. Michaelis constants of approx. 20 μm (formaldehyde), approx. 50 μm (glutathione) and approx. 31 μm (NAD+) were determined for the maize enzyme as well as for FDH partially purified from dog lung. Besides S-hydroxymethylglutathione, pentanol-1, octanol-1, and ω-hydroxyfatty acids served as substrates for both FDH preparations. The unusual substrate specificity indicates that FDH may be involved in the detoxification of long-chain lipid peroxidation products.

Key words: Detoxification of formaldehydeFormal- dehyde dehydrogenase (glutathione-dependent)Protein sequenceZea (formaldehyde dehydrogenase)

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Ulrike Wippermann
    • 1
  • Judith Fliegmann
    • 1
  • Guy Bauw
    • 3
  • Christian Langebartels
    • 1
  • Konrad Maier
    • 2
  • Heinrich Sandermann
    • 1
  1. 1.GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Biochemische Pflanzenpathologie, Ingolstädter Landstraße 1, D-85764 Oberschleißheim, GermanyDE
  2. 2.GSF-Forschungszentrum für Umwelt und Gesundheit, Institut für Inhalationsbiologie, Ingolstädter Landstraße 1, D-85764 Oberschleißheim, GermanyDE
  3. 3.Laboratorium voor Genetica, via the Department of Genetics, affiliated to the Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, B-9000 Gent, BelgiumBE