Planta

, Volume 207, Issue 3, pp 354–361

Cortical cell death, cell proliferation, macromolecular movements and rTip1 expression pattern in roots of rice (Oryza sativa L.) under NaCl stress

  • P. K. Samarajeewa
  • R. A. Barrero
  • C. Umeda-Hara
  • M. Kawai
  • H. Uchimiya
Article

DOI: 10.1007/s004250050492

Cite this article as:
Samarajeewa, P., Barrero, R., Umeda-Hara, C. et al. Planta (1999) 207: 354. doi:10.1007/s004250050492

Abstract.

The mode of action of NaCl in terms of cell proliferation and cell death was examined in seminal roots of rice plants (Oryza sativa L.). Salt/sodium chloride was inhibitory to cell number increase and to cell death in cortical tissue, whereas final cortical cell size was the same as in control roots that were not exposed to NaCl. It seems that NaCl may stimulate the transition phase from cell division to cell elongation. Further analysis of the role of NaCl in the suppression of cortical cell death was confined to a delay in the early stage of cell collapse, which was caused by tonoplast disruption, and plasma-membrane destruction. Sodium chloride did not have any effect on the cell-to-cell movement of macromolecules in the root cortex. In-situ hybridization studies indicated that expression of the gene for tonoplast intrinsic protein (rTip1) was localized predominantly in the epidermal and exodermal cells as well as in metaxylem cells in seminal roots. Upon NaCl treatment, the intensity of rTip1 gene expression was raised in the cortical parenchyma, suggesting that salt plays a role in the rapid onset of cell elongation.

Key words: Aerenchyma Cell death Oryza Root Stress (NaCl) 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • P. K. Samarajeewa
    • 1
  • R. A. Barrero
    • 1
  • C. Umeda-Hara
    • 1
  • M. Kawai
    • 1
  • H. Uchimiya
    • 1
  1. 1.Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, JapanJP
  2. 2.Plant Genetic Resources Centre, P.O. Box 59, Gannoruwa, Peradeniya, Slilanka
  3. 3.Advanced Science Research Center, Japan Atomic Energy Research Institute, Takasaki 370-1292, JapanJP