Planta

, Volume 204, Issue 3, pp 277–287

Cellular dissection of the degradation pattern of cortical cell death during aerenchyma formation of rice roots

Authors

  • M. Kawai
    • Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan
  • P. K. Samarajeewa
    • Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan
  • R. A. Barrero
    • Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan
  • M. Nishiguchi
    • National Institute of Agrobiological Resources, Tsukuba 305, Japan
  • H. Uchimiya
    • Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113, Japan
Article

DOI: 10.1007/s004250050257

Cite this article as:
Kawai, M., Samarajeewa, P., Barrero, R. et al. Planta (1998) 204: 277. doi:10.1007/s004250050257

Abstract.

Cellular events which occur prior to cell collapse were examined in the root cortex of rice (Oryza sativa L.) during aerenchyma formation. Cell collapse started at a specific position in the mid cortex. These cells were distinct in shape from those located towards the periphery. Furthermore, cell collapse was preceded by acidification and the loss of plasma-membrane integrity in cells of the mid cortex. Subsequent death of neighboring cells followed a radial path. Microinjection of molecules of different sizes conjugated with fluorescein isothiocyanate (FITC) showed a molecular exclusion limit of between 9.3 and 19.6 kDa in the root cortex. Furthermore, large molecules, i.e. those around 9.3 kDa, were predominantly transferred in a radial direction, which coincided with the path of sequential cell death.

Key words: AerenchymaCell deathOryzaPlasmodesmaRoot

Copyright information

© Springer-Verlag Berlin Heidelberg 1998