Planta

, Volume 203, Issue 3, pp 349–361

Are the characteristics of betanidin glucosyltransferases from cell-suspension cultures of Dorotheanthus bellidiformis indicative of their phylogenetic relationship with flavonoid glucosyltransferases?

  • Thomas Vogt
  • Elke Zimmermann
  • Rudi Grimm
  • Michael Meyer
  • Dieter Strack
Article

DOI: 10.1007/s004250050201

Cite this article as:
Vogt, T., Zimmermann, E., Grimm, R. et al. Planta (1997) 203: 349. doi:10.1007/s004250050201

Abstract.

Uridine 5′-diphosphoglucose:betanidin 5-O- and 6-O-glucosyltransferases (5-GT and 6-GT; EC 2.4.1) catalyze the regiospecific formation of betanin (betanidin 5-O-β-glucoside) and gomphrenin I (betanidin 6-O-β-glucoside), respectively. Both enzymes were purified to near homogeneity from cell-suspension cultures of Dorotheanthus bellidiformis, the 5-GT by classical chromatographic techniques and the 6-GT by affinity dye-ligand chromatography using UDP-glucose as eluent. Data obtained with highly purified enzymes indicate that 5-GT and 6-GT catalyze the indiscriminate transfer of glucose from UDP-glucose to hydroxyl groups of betanidin, flavonols, anthocyanidins and flavones, but discriminate between individual hydroxyl groups of the respective acceptor compounds. The 5-GT catalyzes the transfer of glucose to the C-4′ hydroxyl group of quercetin as its best substrate, and the 6-GT to the C-3 hydroxyl group of cyanidin as its best substrate. Both enzymes also catalyze the formation of the respective 7-O-glucosides, but to a minor extent. Although the enzymes were not isolated to homogeneity, chromatographic, electrophoretic and kinetic properties proved that the respective enzyme activities were based on the presence of single enzymes, i.e. 5-GT and 6-GT. The N terminus of the 6-GT revealed high sequence identity to a proposed UDP-glucose:flavonol 3-O-glucosyltransferase (UF3GT) of Manihot esculenta. In addition to the 5-GT and 6-GT, we isolated a UF3GT from D. bellidiformis cell cultures that preferentially accepted myricetin and quercetin, but was inactive with betanidin. The same result was obtained with a UF3GT from Antirrhinum majus and a flavonol 4′-O-glucosyltransferase from Allium cepa. Based on these results, the main question to be addressed reads: Are the characteristics of the 5-GT and 6-GT indicative of their phylogenetic relationship with flavonoid glucosyltransferases?

Key words: Betacyanin Dorotheanthus (cell culture) Flavonoid Glucosyltransferase (substrate specificity) 

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Thomas Vogt
    • 1
  • Elke Zimmermann
    • 1
  • Rudi Grimm
    • 2
  • Michael Meyer
    • 3
  • Dieter Strack
    • 1
  1. 1.Institut für Pflanzenbiochemie (IPB), Abteilung Sekundärstoffwechsel, Weinberg 3, D-06120 Halle (Saale), GermanyDE
  2. 2.Hewlett Packard GmbH, Hewlett-Packard-Straße 8, D-76337 Waldbronn, GermanyDE
  3. 3.Institut für Molekulare Biotechnologie (IMB), Biocomputing, Beutenbergstr. 11, D-07708 Jena, GermanyDE

Personalised recommendations