Planta

, Volume 203, Issue 3, pp 320–326

Dynamics of limiting cell wall porosity in plant suspension cultures

Authors

  • Christine Titel
    • Institut für Biologie, Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin, Germany
  • Holger Woehlecke
    • Institut für Biologie, Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin, Germany
  • Izzidin Afifi
    • Institut für Biologie, Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin, Germany
  • Rudolf Ehwald
    • Institut für Biologie, Mathematisch-Naturwissenschaftliche Fakultät I, Humboldt-Universität zu Berlin, Invalidenstrasse 42, D-10115 Berlin, Germany
Article

DOI: 10.1007/s004250050197

Cite this article as:
Titel, C., Woehlecke, H., Afifi, I. et al. Planta (1997) 203: 320. doi:10.1007/s004250050197

Abstract.

Changes in the limiting porosity of cell walls, i.e. the size limit for permeation of neutral molecules through the wall, were studied in several higher-plant cell-suspension cultures. For this purpose, samples of biomass fixed at different cultivation times were investigated using a method based on size-exclusion chromatography of polydisperse dextrans before and after equilibration with the extracted cell clusters. In suspension cultures of Chenopodium album L., Dioscorea deltoidea Wall. and Medicago sativa L., the mean size limit (MSL; critical Stokes' radius for exclusion of neutral polymers from half of the intracellular space) was found to vary between 2.4 and 3.8 nm. It decreased significantly during transition from the growth phase to the stationary phase. In the case of the C. album culture this change was found to be irrespective of whether sucrose in the medium was completely depleted at the end of the growth phase or not. The MSL was kept constant for long periods of the stationary phase if cell viability was maintained by repeated sucrose supplement. In a suspension strain of Triticum aestivum L., the MSL of cell wall permeation was comparatively small (1.75 nm) and remained constant during all cultivation phases. Relations between limiting porosity and cell wall growth, loss of pectic compounds to the medium, cross-linking activities and cell wall stiffening are discussed.

Key words: Cell wall looseningCell wall porosityCell wall stiffeningGrowth (cell wall)Suspension culturePolysaccharide release

Copyright information

© Springer-Verlag Berlin Heidelberg 1997