Planta

, Volume 212, Issue 3, pp 470–474

Enzymic feruloylation of arabinoxylan-trisaccharide by feruloyl-CoA:arabinoxylan-trisaccharide O-hydroxycinnamoyl transferase from Oryza sativa

  • Tomoko Yoshida-Shimokawa
  • Shigeki Yoshida
  • Koichi Kakegawa
  • Tadashi Ishii
Rapid Communication

DOI: 10.1007/s004250000490

Cite this article as:
Yoshida-Shimokawa, T., Yoshida, S., Kakegawa, K. et al. Planta (2001) 212: 470. doi:10.1007/s004250000490

Abstract.

Feruloyl-CoA:arabinoxylan-trisaccharide O-hydroxycinnamoyl transferase, which catalyzes the transfer of ferulic acid from Fer-CoA to arabinoxylan-trisaccharide in the formation of feruloyl arabinoxylan-trisaccharide (Fer-AXX), has been found in an ionically bound fraction and a cytosol fraction of suspension-cultured rice (Oriza sativa L. cv. Nipponbare) cells. Analysis of reaction products by high-performance liquid chromatography showed the formation of product A, which is one of the transfer products having the same retention time as authentic Fer-AXX. Product A was purified by reverse-phase chromatographies to characterize its structure. The isolated product A showed the same ultraviolet spectrum and molecular weight on fast atom bombardment mass spectrometric analysis as those of authentic Fer-AXX. Alakaline saponification of product A released ferulic acid and oligosaccharide. The released oligosaccharide consisted of arabinose and xylose in a molar ratio of 1:2. These results support the identity of product A as feruloylated arabinoxylan-trisaccharide and show the existence of a feruloyltransferase catalyzing the feruloylation of a hemicellulosic fragment.

Key words: ArabinoxylanCell wallFerulic acidFeruloyl-CoAFeruloyltransferaseOryza (cell wall)

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Tomoko Yoshida-Shimokawa
    • 1
  • Shigeki Yoshida
    • 2
  • Koichi Kakegawa
    • 1
  • Tadashi Ishii
    • 1
  1. 1.Forestry and Forest Products Research Institute, P.O. Box 16, Tsukuba Norin Kenkyuu Danchinai, Ibaraki 305-8687, JapanJP
  2. 2.Institute of Applied Biochemistry, University of Tsukuba, Tsukuba-shi, Ibaraki 305-8572, JapanJP