Planta

, Volume 213, Issue 1, pp 132–141

Molecular and physiological characterisation of a 14-3-3 protein from lily pollen grains regulating the activity of the plasma membrane H+ ATPase during pollen grain germination and tube growth

  • Heidi Pertl
  • Martin Himly
  • Renate Gehwolf
  • Rosa Kriechbaumer
  • Doris Strasser
  • Wolfgang Michalke
  • Klaus Richter
  • Fatima Ferreira
  • Gerhard Obermeyer

DOI: 10.1007/s004250000483

Cite this article as:
Pertl, H., Himly, M., Gehwolf, R. et al. Planta (2001) 213: 132. doi:10.1007/s004250000483

Abstract.

A 14-3-3 protein has been cloned and sequenced from a cDNA library constructed from mRNAs of mature pollen grains of Lilium longiflorum Thunb. Monoclonal antibodies (MUP 5 or MUP 15) highly specific against 14-3-3 proteins recognised a 30-kDa protein in the cytoplasmic fraction of many various lily tissues (leaves, bulbs, stems, anther filaments, pollen grains, stigmas) and in other plants (Arabidopsis seedlings, barley recombinant 14-3-3). In addition, 14-3-3 proteins were detected in a microsomal fraction isolated from pollen grains and tubes, and the amount of membrane-bound 14-3-3 proteins as well as the amount of the plasma membrane (PM) H+ ATPase increased during germination of pollen grains and tube growth. No change was observed in the cytoplasmic fraction. A further increase in the amount of 14-3-3 proteins in the microsomal fraction was observed when pollen grains were incubated in germination medium containing 1 μM fusicoccin (FC) whereas the number of 14-3-3s in the cytoplasmic fraction decreased. Fusicoccin also protected membrane-bound 14-3-3 proteins from dissociation after washing with the chaotropic salt KI. Furthermore, FC stimulated the PM H+ ATPase activity, the germination frequency and the growth rate of pollen tubes, thus indicating that a modulation of the PM H+ ATPase activity by interaction with 14-3-3 proteins may regulate germination and tube growth of lily pollen.

Key words: Fusicoccin H+ ATPase Lilium (pollen) Pollen 14-3-3 Protein Tip growth 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Heidi Pertl
    • 1
  • Martin Himly
    • 2
  • Renate Gehwolf
    • 1
  • Rosa Kriechbaumer
    • 1
  • Doris Strasser
    • 1
  • Wolfgang Michalke
    • 3
  • Klaus Richter
    • 2
  • Fatima Ferreira
    • 2
  • Gerhard Obermeyer
    • 1
  1. 1. Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, AustriaAT
  2. 2. Institut für Allgemeine Biologie und Genetik, Universität Salzburg, Hellbrunnerstrasse 34, 5020 Salzburg, AustriaAT
  3. 3. Institut für Biologie III, Universität Freiburg, Schänzlestrasse1, 79104 Freiburg, GermanyDE

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