Planta

, Volume 212, Issue 4, pp 635–639

Enhanced disease resistance conferred by expression of an antimicrobial magainin analog in transgenic tobacco

  • Qingshun Li
  • Christopher B. Lawrence
  • Hong-Yan Xing
  • Ruth A. Babbitt
  • W. Troy Bass
  • Indu B. Maiti
  • Nicholas P. Everett
Rapid Communication

DOI: 10.1007/s004250000480

Cite this article as:
Li, Q., Lawrence, C., Xing, H. et al. Planta (2001) 212: 635. doi:10.1007/s004250000480

Abstract.

Magainins are a group of short peptides originally isolated from frog skin and thought to function as a natural defense mechanism against infection due to their antimicrobial properties. The engineered magainin analog peptide Myp30 was found to inhibit spore germination of the oomycete, Peronospora tabacina (Adam) in vitro, and the growth of a bacterial pathogen Erwinia carotovora subsp. carotovora (Jones). Transgenic tobacco (Nicotiana tabacum L.) plants expressing Myp30 were evaluated for resistance to these pathogens. The expression of the peptide only to an extracellular location resulted in significant reduction in sporulation and lesion size due to P. tabacina infection. A significant increase in resistance to the bacterial pathogen was also observed regardless of the targeting location of the peptide.

Key words: Antimicrobial peptideDisease resistanceErwiniaMagaininNicotianaPeronospora

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Qingshun Li
    • 1
  • Christopher B. Lawrence
    • 1
  • Hong-Yan Xing
    • 1
  • Ruth A. Babbitt
    • 1
  • W. Troy Bass
    • 1
  • Indu B. Maiti
    • 1
  • Nicholas P. Everett
    • 2
  1. 1. THRI, University of Kentucky, Lexington, KY 40546-0236, USAUS
  2. 2. Interlink Biotechnologies LLC, Auburn, CA 95603, USAUS