Planta

, Volume 212, Issue 5, pp 880–887

Metabolic response of potato plants to an antisense reduction of the P-protein of glycine decarboxylase

  • Dieter Heineke
  • Natalia Bykova
  • Per Gardeström
  • Hermann Bauwe

DOI: 10.1007/s004250000460

Cite this article as:
Heineke, D., Bykova, N., Gardeström, P. et al. Planta (2001) 212: 880. doi:10.1007/s004250000460

Abstract.

Potato (Solanum tuberosum L. cv. Desiré) plants with reduced amounts of P-protein, one of the subunits of glycine decarboxylase (GDC), have been generated by introduction of an antisense transgene. Two transgenic lines, containing about 60–70% less P-protein in the leaves compared to wild-type potato, were analysed in more detail. The reduction in P-protein amount led to a decrease in the ability of leaf mitochondria to decarboxylate glycine. Photosynthetic and growth rates were reduced but the plants were viable under ambient air and produced tubers. Glycine concentrations within the leaves were elevated up to about 100-fold during illumination. Effects on other amino acids and on sucrose and hexoses were minor. Nearly all of the glycine accumulated during the day was metabolised during the following night. The data suggest that the GDC operates far below substrate saturation under normal conditions thus allowing a flexible and fast response to changes in the environment.

Key words: Antisense effectsGlycine decarboxylasePhotorespirationPhotosynthesisSolanum (glycine decarboxylase)Transgenic potato

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Dieter Heineke
    • 1
  • Natalia Bykova
    • 2
  • Per Gardeström
    • 2
  • Hermann Bauwe
    • 3
  1. 1.Albrecht-von-Haller-Institut für Pflanzenwissenschaften, Abteilung Biochemie der Pflanze, Universität Göttingen, Untere Karspüle 2, 37073 Göttingen, GermanyDE
  2. 2.Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187 Umeå, SwedenSE
  3. 3.Institut für Molekulare Physiologie und Biotechnologie, Abteilung Pflanzenphysiologie, Universität Rostock, Doberaner Str. 143, 18051 Rostock, GermanyDE