Planta

, Volume 212, Issue 3, pp 348–358

Altered pectin composition in primary cell walls of korrigan, a dwarf mutant of Arabidopsis deficient in a membrane-bound endo-1,4-β-glucanase

Authors

  • Isabelle His
    • UMR CNRS 6037, Centre de Microscopie Electronique, IFRMP 23, Université de Rouen, 76821 Mont-Saint-Aignan Cedex, France
  • Azeddine Driouich
    • UMR CNRS 6037, Centre de Microscopie Electronique, IFRMP 23, Université de Rouen, 76821 Mont-Saint-Aignan Cedex, France
  • Frédéric Nicol
    • Laboratoire de Biologie Cellulaire, Institut National de Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France
  • Alain Jauneau
    • Laboratoire Signaux et Messages Cellulaires chez les Végétaux, IFR 40 Pôle de Biotechnologie Végétale, 24 Chemin de Borde Rouge, B.P. 17 Auzeville, 31326 Castanet-Tolosan, France
  • Herman Höfte
    • Laboratoire de Biologie Cellulaire, Institut National de Recherche Agronomique, Route de Saint-Cyr, 78026 Versailles Cedex, France

DOI: 10.1007/s004250000437

Cite this article as:
His, I., Driouich, A., Nicol, F. et al. Planta (2001) 212: 348. doi:10.1007/s004250000437

Abstract.

Korrigan (kor) is a dwarf mutant of Arabidopsis thaliana (L.) Heynh. that is deficient in a membrane-bound endo-1,4-β-glucanase. The effect of the mutation on the pectin network has been studied in kor by microscopical techniques associated with various probes specific for different classes of pectic polysaccharides. The localisation of native crystalline cellulose was also examined using the cellobiohydrolase I-gold probe. The investigations were focused on the external cell walls of the epidermis, a cell layer that, in a number of plant species, has been shown to be growth limiting. Anionic sites associated with pectic polymers were quantified using the cationic gold probe. Homogalacturonans were quantified using polyclonal anti-polygalacturonic acid/rhamnogalacturonan I antibodies recognising polygalacturonic acid, and monoclonal JIM7 and JIM5 antibodies recognising homogalacturonans with a high or low degree of methyl-esterification, respectively. Rhamnogalacturonans were quantified with two monoclonal antibodies, LM5, recognising β-1,4 galactan side chains of rhamnogalacturonan I, and CCRCM2. Our results show a marked increase in homogalacturonan epitopes and a decrease in rhamnogalacturonan epitopes in kor compared to the wild type. A substantial decrease in cellobiohydrolase I-gold labelling was also observed in the mutant cell walls. These findings demonstrate that a deficiency in an endo-1,4-β-glucanase, which is in principle not directly implicated in pectin metabolism, can induce important changes in pectin composition in the primary cell wall. The changes indicate the existence of feedback mechanisms controlling the synthesis and/or deposition of pectic polysaccharides in primary cell walls.

Key words:Arabidopsis (mutantpectin)CelluloseCell wallkor mutant (Arabidopsis)Pectin (immunocytochemistry)Polysaccharide

Copyright information

© Springer-Verlag Berlin Heidelberg 2001