Planta

, Volume 211, Issue 4, pp 484–492

The seed coat-specific expression of a subtilisin-like gene, SCS1, from soybean

  • Anthea K. Batchelor
  • Kim Boutilier
  • S. Shea Miller
  • Hélène Labbé
  • LuAnne Bowman
  • Ming Hu
  • Douglas A. Johnson
  • Mark Gijzen
  • Brian L.A. Miki

DOI: 10.1007/s004250000320

Cite this article as:
Batchelor, A., Boutilier, K., Miller, S. et al. Planta (2000) 211: 484. doi:10.1007/s004250000320

Abstract.

A seed coat-specific gene, SCS1 (Seed Coat Subtilisin 1), from soybean, Glycine max [L.] Merill, has been identified and studied. The gene belongs to a small family of genes with sequence similarity to the subtilisins, which are serine proteases. Northern blot analysis showed that SCS1 RNA accumulates to maximal levels in seed coats at 12 days post anthesis, preceding the final stages of seed coat differentiation. The SCS1 RNA was not found in other tissues including embryos, seed pods, flowers, stems, roots or leaves. In-situ hybridization studies confirmed the temporal pattern of expression observed by Northern blot analysis and further revealed a restricted pattern of RNA accumulation in thick-walled parenchyma cells of the seed coats. These cells are important in the apoplastic translocation of nutrients en route to the embryo from the vascular tissues. The tissue-specific subtilisin-like gene may be required for regulating the differentiation of the thick-walled parenchyma cells.

Key words:Glycine (seed coat) – Seed coat – Parenchyma (thick walled) – Subtilisin-like gene

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Anthea K. Batchelor
    • 3
  • Kim Boutilier
    • 1
  • S. Shea Miller
    • 1
  • Hélène Labbé
    • 1
  • LuAnne Bowman
    • 1
  • Ming Hu
    • 1
  • Douglas A. Johnson
    • 3
  • Mark Gijzen
    • 2
  • Brian L.A. Miki
    • 1
  1. 1.Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario K1A 0C6, CanadaCA
  2. 2.Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, CanadaCA
  3. 3.Biology Department, University of Ottawa, Ottawa, Ontario K1N 6N5, CanadaCA