Planta

, Volume 211, Issue 2, pp 256–264

Identification and partial characterization of proteins and proteoglycans encrusting the secondary cell walls of flax fibres

  • Raynald Girault
  • Isabelle His
  • Christine Andeme-Onzighi
  • Azeddine Driouich
  • Claudine Morvan

DOI: 10.1007/s004250000281

Cite this article as:
Girault, R., His, I., Andeme-Onzighi, C. et al. Planta (2000) 211: 256. doi:10.1007/s004250000281

Abstract.

 Four proteins were isolated from depectinised elementary fibres of flax (Linum usitatissimum L.), using either alkali or cellulase digestion treatments. All the four proteins were characterized by a deficiency or low contents of hydroxyproline and by high levels of glutamic acid/glutamine and/or aspartic acid/asparagine. The two proteoglycans solubilized with cellulase strongly reacted with β-glucosyl Yariv reagent but not with α-glucosyl Yariv reagent and contained appreciable amounts of alanine, glycine, serine and threonine, suggesting a relationship with cell wall hydroxyproline-deficient arabinogalactan-proteins. The two alkali-extracted proteins did not show any reaction with β-glucosyl Yariv dye. Due to the harsh treatment, they might only partially represent the original proteins. Due to its high level of glycine (41%), one of these proteins might be classified as a glycine-rich protein. The latter polypeptide, of low molecular molar mass, contained 14.6% leucine and might consist of a domain related to leucine-rich proteins. The data show that these proteins and arabinogalactan-protein-like proteoglycans were strongly associated with the secondary walls of flax fibres. Their presence in small amounts (0.1–0.4%), raises the problem of their putative structural role.

Key words: Arabinogalactan-proteinGlycine-rich proteinHydroxyproline-deficient proteinLeucine-rich proteinLinum (cell wall)Secondary cell wallYariv phenylglycosides

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Raynald Girault
    • 1
  • Isabelle His
    • 1
  • Christine Andeme-Onzighi
    • 1
  • Azeddine Driouich
    • 1
  • Claudine Morvan
    • 1
  1. 1.SCUEOR ESA 6037 CNRS, University of Rouen, 76821 Mont Saint Aignan cedex, FranceFR