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Emerging technologies for non-invasive quantification of physiological oxygen transport in plants

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Abstract

Oxygen plays a critical role in plant metabolism, stress response/signaling, and adaptation to environmental changes (Lambers and Colmer, Plant Soil 274:7–15, 2005; Pitzschke et al., Antioxid Redox Signal 8:1757–1764, 2006; Van Breusegem et al., Plant Sci 161:405–414, 2001). Reactive oxygen species (ROS), by-products of various metabolic pathways in which oxygen is a key molecule, are produced during adaptation responses to environmental stress. While much is known about plant adaptation to stress (e.g., detoxifying enzymes, antioxidant production), the link between ROS metabolism, O2 transport, and stress response mechanisms is unknown. Thus, non-invasive technologies for measuring O2 are critical for understanding the link between physiological O2 transport and ROS signaling. New non-invasive technologies allow real-time measurement of O2 at the single cell and even organelle levels. This review briefly summarizes currently available (i.e., mainstream) technologies for measuring O2 and then introduces emerging technologies for measuring O2. Advanced techniques that provide the ability to non-invasively (i.e., non-destructively) measure O2 are highlighted. In the near future, these non-invasive sensors will facilitate novel experimentation that will allow plant physiologists to ask new hypothesis-driven research questions aimed at improving our understanding of physiological O2 transport.

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Acknowledgments

The authors also acknowledge the UF Excellence Award and the IFAS Early Career Award (CRIS No. 005062) for funding (McLamore). A special thanks to PIKL (Baltimore, MD) for help with graphic images.

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Authors and Affiliations

  1. Agricultural and Biological Engineering Department, University of Florida, Gainesville, USA

    P. Chaturvedi, M. Taguchi, S. L. Burrs & E. S. McLamore

  2. Department of Biology, University of Florida, Gainesville, USA

    B. A. Hauser

  3. Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, USA

    W. W. A. W. Salim

  4. Birck Nanotechnology Center/Bindley Bioscience Center Physiological Sensing Facility, Purdue University, West Lafayette, USA

    W. W. A. W. Salim

  5. U.S. Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington, DC, USA

    J. C. Claussen

  6. College of Science, George Mason University, Fairfax, USA

    J. C. Claussen

Authors
  1. P. Chaturvedi
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  2. M. Taguchi
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  3. S. L. Burrs
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  4. B. A. Hauser
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  5. W. W. A. W. Salim
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  6. J. C. Claussen
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  7. E. S. McLamore
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Corresponding author

Correspondence to E. S. McLamore.

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Chaturvedi, P., Taguchi, M., Burrs, S.L. et al. Emerging technologies for non-invasive quantification of physiological oxygen transport in plants. Planta 238, 599–614 (2013). https://doi.org/10.1007/s00425-013-1926-9

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  • DOI: https://doi.org/10.1007/s00425-013-1926-9

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